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Overview
Flow cytometry is widely used for disease-specific assessments and immunophenotyping of immune cell populations. This evaluation helps in diagnosis of immune deficiencies. Determining lymphocyte subsets can generate more insights of the immune system than a complete blood count (CBC).
Average frequency of Leukocytes and lymphocyte subpopulations in a complete blood count
BD Multitest™ 6-Color TBNK reagent with optional BD Trucount™ Tubes is a six-color direct immunofluorescence reagent for use in identifying and determining the percentages and absolute counts of T, B, and natural killer (NK) cells, as well as the CD4+ and CD8+ subpopulations of T cells, in peripheral blood on a BD flow cytometer.
Determining percentages or absolute counts of CD3+CD4+ T lymphocytes is used in monitoring human immunodeficiency virus (HIV)-infected individuals. Individuals with HIV typically exhibit a steady decrease of CD3+CD4+ T-lymphocyte absolute counts as the infection progresses.1
Determining percentages or absolute counts of CD3+, CD3+CD4+, or CD3+CD8+ T lymphocytes or CD19+ B lymphocytes is used to characterize or monitor some forms of immune deficiency and autoimmune diseases.1,2
Determining percentages or absolute counts of CD3– and CD16+ and/or CD56+ NK lymphocytes is used in immunological assessment of hematologically-normal subjects or patients having, or suspected of having, immune deficiency or other immune-mediated diseases.1,3
Why BD Multitest™ 6-Color
TBNK Kit?
- Performs lymphocyte enumeration of mature T, B and NK cells in a single tube
- Provides the option of absolute counts when paired with BD Trucount™ Tubes
- Works in a fully integrated system with BD FACSDuet™ Sample Preparation System for automated sample preparation allwoing a complete automated workflow from sample to answer, reducing hands-on time and error-prone steps.
- Can be used with BD FACS™ Universal Loader for automated sample acquisition on BD FACSLyric™
- CE-IVD assay modules with built-in templates and automated gating to work on BD FACSLyric™ instrument software for setup, QC, acquisition analysis and reporting. Seamless integration of data reporting of test and patient results through bidirectional LIS connectivity.
Features
The BD Multitest™ 6-Color TBNK Kit Simplifies Your Workflow and Increases Productivity
- An easy-to-use Solution
With its single-tube format, the BD Multitest™ 6-Color TBNK reagent decreases cost by reducing the number of tubes and sample processing time. The reagent includes a cocktail of multiple fluorescently labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining.
- Accurate cell counts with BD Trucount™ Tubes
BD Trucount™ Tubes help to ensure more accurate cell counts - requires no additional pipetting steps, precise and reproducible. The tubes contain a lyophilized pellet of fluorescent beads that serves as an internal standard. BD FACSuite™ Clinical Application automatically calculates lymphocyte absolute counts and subset percentages.
- Improve safety, reduce hands-on time
The BD Multitest™ 6-Color TBNK reagent employs a no-wash formula that reduces tube handling. Processing fewer tubes in less time may decrease potential exposure to biohazardous material, improving safety for laboratory personnel.
Increase productivity
Together with the BD FACSLyric™ cell analyzer and BD FACSuite™ software, the BD FACS™ Universal Loader option and the BD FACSDuet™ Sample Preparation System, the BD Multitest™ 6-Color TBNK reagent provides a fully automated, standardized and efficient workflow for lymphocyte enumeration to best suit your routine immunophenotyping needs.
Applications
The BD Multitest™ 6-Color TBNK reagent with Trucount™ tube for accurate and reliable enumeration of mature T, B and NK cells.
A single tube, single platform CE- IVD assay utilizing BD Trucount™ Tube technology
A hematological normal adult sample with BD Multitest™ 6 Color TBNK in a BD Trucount™ Tube was acquired on a BD FACSLyric™ Flow Cytometer.
BD FACSLyric™ laboratory report showing data collected with BD Trucount™ Tubes. See the BD FACSLyric™ Clinical Reference System, which provides information on gating and troubleshooting.
Resources
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Instruction for Use
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Protocols
BD FACSDuet™ Sample Preparation System, BD FACS™ Universal Loader, BD FACSLyric™ Flow Cytometer with the BD FACSuite™ Clinical and BD FACSuite™ applications and BD FACSuite™ Clinical BD Multitest™ 6-Color Assays are in vitro diagnostic medical devices bearing a CE mark.
BD Multitest™ 6-Color TBNK and the optional BD Trucount™ Tubes are in vitro diagnostic medical devices bearing a CE mark and are CE certified by BSI Group The Netherlands B.V. (Notified Body Number = 2797).
BD Flow Cytometers and BD FACSDuet™ Sample Preparation System are Class 1 Laser Products.
References:
- Cossarizza A, De Biasi S, Gibellini L, et al. Cytometry, immunology, and HIV infection: three decades of strong interactions. Cytometry. Part A: the Journal of the International Society for Analytical Cytology. 2013;83(8):680-691.
- Hanson IC, Shearer WT. Ruling out HIV infection when testing for severe combined immunodeficiency and other T-cell deficiencies. J Allergy Clin Immunol. 2012;129(3):875-876.e875
- Oliveira JB, Fleischer TA. Molecular- and flow cytometry-based diagnosis of primary immunodeficiency disorders. Current Allergy and Asthma Reports. 2010:10(6):460-467.