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BD Accuri™ C6 Plus Personal Flow Cytometer

The cell analyzer with capabilities to bring flow cytometry even more within reach for new and experienced researchers alike

Overview Features Performance Applications Resources and Training

Overview

The BD Accuri™ C6 Plus Flow Cytometer, with its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb and operational simplicity, supports a wide variety of applications including immunology, cell and cancer biology, plant and microbiology and industrial applications. This portable and compact cell analyzer holds the analytical power of a cytometer twice its size. Backed by 23,000 citations to date*, the BD Accuri™ C6 Plus System enables you to go beyond your current research potential.

 

Get kits and templates for the BD Accuri™ C6 Plus Personal Flow Cytometer (also work with the discontinued BD Accuri™ C6 Personal Flow Cytometer).

 

*Google Scholar™: https://scholar.google.com/scholar?hl=en&as_sdt=0%2C5&q=Accuri&btnG= ; Accessed June 2020.

 
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Features

The BD Accuri™ C6 Plus Flow Cytometer Advantages

Sensitive, reliable and capable

  • Reliable instrument performance with automated QC
  • Volumetric counting and continuous sampling

Simple: easy to learn, use, maintain and teach

  • Quick and easy instrument setup
  • Simplified workflow with BD quality reagents and kits
  • Walkaway convenience with automated sample loader

Compact and transportable

  • Small footprint for easy transportation between labs

Intuitive and easy-to-learn BD Accuri™ C6 Plus Software

  • Intuitive user interface
  • Easy to learn and data can be collected and analyzed in less than an hour
  • Clearly visible software options and instrument controls

The BD Accuri™ C6 Plus System Accurately Monitors the Sample Volume Pulled Per Run

Fluidics

  • A unique, nonpressurized, peristaltic pump system drives the fluidics
  • The system can calculate absolute counts or sample concentration per µL without the use of counting beads

 

The optional BD CSampler™ Plus Software accessory offers reliable, easy-to-use automation while adding minimal footprint:

  • 48-well plates
  • 96-well plates
  • 96-deep-well plates
  • 24-tube rack (included) for standard 12 x 75-mm tubes

The BD Accuri™ C6 Plus Flow Cytometer, Combined with BD® Kits and Templates, Provides Better Insight and Easier Workflow

As easy as cell analysis is going to get

Apply the power of flow cytometry to your research with ready-to-go reagent kits and free software templates specific to your studies.

Preconfigured kits include fluorescent antibodies, buffer systems and other reagents needed for acquisition and analysis. 

Kits and templates are available for various applications:

Immunophenotyping, apoptosis, cell cycle, microbial counting, intracellular cytokine studies, stem cell analysis and more.

The BD Accuri™ C6 Plus Cell Analyzer Provides Simplicity and Sensitivity on Your Benchtop 

 

Optics

  • Equipped with a blue and red laser, two light scatter detectors and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy5.5 and APC
  • Easy to use with no need to adjust detector voltage
  • Data are digitally collected over a wide dynamic range and are fully available to you as needed, eliminating the risk of data loss due to incorrect settings
  • Software functions such as Zoom and VirtualGain allow visualization of data at any scale, so you can precisely set gates and regions
  • Extraordinary fluorescence sensitivity with optical and fluidic design provide the ability to withstand rugged conditions. Provided the system is anchored, it can run samples even if the benchtop is in motion, such as aboard a ship
Excitation Wavelength Fluorochromes/Dyes Standard Filter Optional Filter
488 nm FITC, BB515, Alexa Fluor™ 488, CFSE 533/30 -
GFP 533/30 510/15
JC-1, Fluo-4 AM, SYBR™Green  533/30 -
YFP  533/30 540/20
PE, JC-1, BD™ MitoStatus TMRE  585/40 -
OFP  585/40 565/20
Propidium Iodide  585/40, 670 LP 610/20
BD Horizon™ PE-CF594  670 LP 670 LP>610/20
RFP, mCherry, dsRed  670 LP 610/20
PerCP, PE-Cy5  670 LP -
PerCP-Cy5.5  670 LP 670 LP>-
7-AAD  670 LP 670 LP>780/60
PE Cy7  670 LP 780/60
640 nm APC, BD™ MitoStatus Red, Alexa Fluor™ 647 675/25 LP -
APC-H7, APC-Cy7  670 LP 780/60

 

 

Performance

Adaptable optics, with many filter, laser and configuration options, and the unique fluidics system provide flexibility in the fluorochrome combinations you can analyze and automation for direct counting of cells

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Identification of blood cell populations in a single tube using four colors

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Bacterial viability on the BD Accuri™ C6 Plus System.

Applications

Trusted Research Reagents

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BD Accuri™ Flow Cytometers Enable Immunology Research and Immunophenotyping of Cell Populations

Several features of BD Accuri™ Flow Cytometers make them ideal platforms for differential analysis of human peripheral blood samples.
 

  • Fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification
  • The broad dynamic range makes it easy to analyze populations as varied in size as platelets and eosinophils in the same data file
  • The easy-to-use software Zoom tool allows precise control when setting gates
  • Direct volume measurement allows direct calculation of the cell concentration per µL for each population of interest

View Technical Bulletin describing the identification of human peripheral blood cell populations with the BD Accuri™ Flow Cytometer.

BD Accuri™ Flow Cytometers Have Been Used in Cell and Cancer Biology Applications

With two lasers and four fluorescence detectors, the BD Accuri™ Personal Flow Cytometers bring versatility and convenience to your benchtop. Two additional features can further simplify your research:

 

Absolute counts: Cell concentration can be calculated directly from BD Accuri™ Software statistics tables without the addition of counting beads.

 

Kits and templates: Include needed reagents and buffers to assess apoptosis, cell cycle and proliferation and DNA damage. Free BD Accuri™ Software templates simplify data collection and analysis.

­Technical Bulletin: Multiple Methods for Detecting Apoptosis on the BD Accuri Flow Cytometer
Product Information Sheet: BD Apoptosis Kits and Templates
Product Information Sheet: BD Cell Cycle/DNA Kits and Templates

Use of BD Accuri™ Flow Cytometers in Microbiology Applications
  • Microbe counts and concentrations can be calculated rapidly, directly and automatically from the software statistics tables without the addition of counting beads
  • If microbes are transfected with green, yellow, red or other fluorescent proteins, they can be detected and clearly differentiated from non-transfected populations
  • BD Accuri™ Systems are ideal for monitoring drinking water because of their transportability, open fluidics systems and ability to determine sample volume and calculate cell concentrations directly. A free software template for the Eawag protocol simplifies setup and analysis

Technical Bulletin: A Novel, Transportable Flow Cytometer Facilitates Algal Quantification in Cultures and Environmental Samples
Product Information Sheet: BD® Cell Viability Kit
Product Information Sheet: BD Accuri™ Eawag Water Quality Template

Industrial Applications of the BD Accuri™ Flow Cytometer
  • BD Accuri™ Flow Cytometers are compact and rugged and can be transported into labs, processing facilities and even into the field
  • The open fluidics system allows it to connect easily to bioreactors and fermentation units for automatic sampling and continuous culture monitoring
  • The light scatter signals can be used to discriminate cells and fluorescence signals to measure cell viability, vitality and other important cell characteristics
  • The instruments also interface smoothly with automated bioreactor sampling systems so that samples can be taken continuously and analyzed automatically

White Paper: Bioprocess Monitoring with the BD Accuri™ Flow Cytometer
Access other White Papers on Assessing water quality, Continuous measurement of intracellular calcium, Determining cell concentrations by direct volume, Life science applications of personal flow cytometry, Sowing the seeds of knowledge: the integration of practical flow cytometry into undergraduate education, Rapid and accurate analysis of the nuclear DNA contents of flowering plants.

BD Accuri™ C6 Plus Personal Flow Cytometer

The cell analyzer with capabilities to bring flow cytometry even more within reach for new and experienced researchers alike

Request a Quote
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Resources and Training
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Class 1 Laser Product.

All reagents and kits are compatible with both the BD Accuri C6 Plus and BD Accuri C6 Flow Cytometer Systems. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. Data were generated on either the BD Accuri C6 Plus or the BD Accuri C6 System as indicated in figure legends.

BD Accuri C6 System (32-bit) Software is not forward compatible with BD Accuri C6 Plus (64-bit) Software and the FCS 3.1 file format.

Alexa Fluor and SYBR are trademarks of Thermo Fisher Scientific. Dell and OptiPlex are trademarks of Dell Inc. Google Scholar is a trademark of Google LLC. Microsoft and Excel are trademarks of Microsoft Corporation. Cy is a trademark of GE Healthcare. Cy dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.