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Purified Mouse Anti-Rat CD28
Purified Mouse Anti-Rat CD28

Flow cytometric analysis of CD28 expression on rat thymocytes. LEW thymocytes were stained with FITC Mouse Anti-Rat CD3 (Cat. No. 559975/554832) alone (Left Panel) or costained with Purified Mouse Anti-Rat CD28 (Cat. No. 559982; Right Panel), followed with PE Rat Anti-Mouse IgG1 (Cat. No. 550083). Two-color contour plots were derived from gated events with the forward and side light-scattering characteristics of intact thymocytes. Flow cytometry was performed on a BD FACScan™ system.

Flow cytometric analysis of CD28 expression on rat thymocytes. LEW thymocytes were stained with FITC Mouse Anti-Rat CD3 (Cat. No. 559975/554832) alone (Left Panel) or costained with Purified Mouse Anti-Rat CD28 (Cat. No. 559982; Right Panel), followed with PE Rat Anti-Mouse IgG1 (Cat. No. 550083). Two-color contour plots were derived from gated events with the forward and side light-scattering characteristics of intact thymocytes. Flow cytometry was performed on a BD FACScan™ system.

Product Details
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BD Pharmingen™
Cd28; CD28RNA
Rat (QC Testing)
Mouse BALB/c IgG1, κ
Rat CD28-transfected cell line
Flow cytometry (Routinely Tested), (Co)-stimulation, Immunoprecipitation (Reported)
0.5 mg/ml
AB_397409
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559982 Rev. 11
Antibody Details
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JJ319

The JJ319 monoclonal antibody specifically recognizes CD28, which is expressed on virtually all T lymphocytes bearing αβ T-cell receptors (TCR), on most γδ TCR-bearing T cells, and on a subset of NK cells. In the thymus, CD28 expression is developmentally regulated during the maturation of αβ TCR-bearing T cells. CD28 is a costimulatory receptor required for activation of T cells; its ligands include CD80 (B7-1) and CD86 (B7-2). Soluble JJ319 mAb costimulates the proliferative responses and IL-2 production of CD4+ and CD8+ T cells activated by anti-αβ-TCR mAb R73 (Cat. no. 554910). The alternate anti-rat CD28 mAb JJ316 (Cat. No. 554992) is capable of directly stimulating T cells in vitro and in vivo.

559982 Rev. 11
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559982 Rev.11
Citations & References
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Development References (4)

  1. Bluestone JA. New perspectives of CD28-B7-mediated T cell costimulation. Immunity. 1995; 2(6):555-559. (Biology). View Reference
  2. Mitnacht R, Tacke M, Hunig T. Expression of cell interaction molecules by immature rat thymocytes during passage through the CD4+8+ compartment: developmental regulation and induction by T cell receptor engagement of CD2, CD5, CD28, CD11a, CD44 and CD53. Eur J Immunol. 1995; 25(2):328-332. (Biology). View Reference
  3. Tacke M, Clark GJ, Dallman MJ, Hunig T. Cellular distribution and costimulatory function of rat CD28. Regulated expression during thymocyte maturation and induction of cyclosporin A sensitivity of costimulated T cell responses by phorbol ester. J Immunol. 1995; 154(10):5121-5127. (Immunogen: (Co)-stimulation, Immunoprecipitation). View Reference
  4. Tacke M, Hanke G, Hanke T, Hunig T. CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 1997; 27(1):239-247. (Clone-specific: (Co)-stimulation, Immunoprecipitation). View Reference
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559982 Rev. 11

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.