PE Rat Anti-Mouse IgG1

Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info

BD Pharmingen™ PE Rat Anti-Mouse IgG1

Name: PE Rat Anti-Mouse IgG1
Brand: BD Pharmingen™
SKU: 550083

Clone A85-1

(RUO)

Flow cytometric analysis of CD22.2 expression by mouse splenocytes using PE Rat Anti-Mouse IgG1 as a second step. BALB/c mouse splenic leucocytes were stained with either Purified Mouse IgG1, κ Anti-Mouse CD22.2 antibody (clone Cy34.1; solid line histogram) or with no antibody (for background staining control; dashed line histogram). After washing, the cells were stained with PE Rat Anti-Mouse IgG1 antibody (Cat. No. 550083) as the second step at 1 µg/test. The fluorescence histogram showing CD22.2 expression (or control staining) as detected by PE Rat Anti-Mouse IgG1 antibody was derived from gated events with the forward and side light-scatter characteristics of intact splenocytes. Flow cytometry and analysis was performed using a BD™ LSR II Flow Cytometer System and FlowJo™ software.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4

Reactivity: Mouse (QC Testing)

Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ

Immunogen: Pooled Mouse IgG1

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 16017

RRID: AB_393553

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

For flow cytometric detection of intracytoplasmic IgG1, we recommend FITC-conjugated mAb A85-1 (Cat. No. 553443).

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

550083 Rev. 7

Antibody Details

A85-1

The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody. A suspension of pooled mouse IgG1 was used as the source of immunogen.

550083 Rev. 7

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

550083 Rev.7

Citations & References

Development References (3)

Bhattacharya D, Lee DU, Sha WC. Regulation of Ig class switch recombination by NF-kappaB: retroviral expression of RelB in activated B cells inhibits switching to IgG1, but not to IgE. Int Immunol. 2002; 14(9):983-991. (Clone-specific: Flow cytometry). View Reference
Honjo T, Obata M, Yamawaki-Katoaka Y, et al. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene. Cell. 1979; 18(2):559-568. (Biology). View Reference
Ozaki K, Spolski R, Ettinger R, et al. Regulation of B cell differentiation and plasma cell generation by IL-21, a novel inducer of Blimp-1 and Bcl-6. J Immunol. 2004; 173(9):5361-5371. (Clone-specific: Flow cytometry). View Reference

550083 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.