The C8 monoclonal antibody specifically recognizes C57BL/6 mouse CD161 antigen-like family member F (CD161f ). CD161f is also known as Natural killer cell surface protein NKR-P1F (Nkrp1f), and is encoded by Klrb1f (Killer cell lectin-like receptor subfamily B member 1F). CD161f is expressed on natural killer (NK) cells, and some dendritic cells and activated lymphocytes. CD161f binds to C-type lectin related protein c, d, and g (Clr-c, d, g) which are expressed on several cell types including dendritic cells, B cells, and activated T cells. CD161f-mediated signaling can reportedly costimulate T cell proliferation and IL-2 production, and may play a role in dendritic cell maturation.
The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max near 407 nm and Em Max near 421 nm, BD Horizon BV421 can be excited by the violet laser (405 nm) and detected with a 450/50 nm filter. BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue™ conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon V450 cannot be used simultaneously.