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A14020_610069_image1.png

Western blot analysis of Annexin II on a A431 lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- Annexin II antibody.

A14020_610069_image2.png

Immunofluorescence staining of human fibroblasts.

A14020_610069_image1.png A14020_610069_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of Annexin II on a A431 lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of the anti- Annexin II antibody.

Immunofluorescence staining of human fibroblasts.

Product Details
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BD Transduction Laboratories™
Lipocortin II, Calpactin I
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Human Annexin II aa. 123-328
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)
36 kDa
250 µg/ml
AB_397480
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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610068 Rev. 1
Antibody Details
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5/Annexin II

Annexin II (p36), originally discovered as a substrate for the src oncogene, is one of the most studied members of the annexin family of calcium-dependent phospholipid-binding proteins. It exists in cells as either a monomer or a heterotetramer complexed with p11, an S100-related protein. In vitro, it has been found that unphosphorylated annexin II is capable of causing aggregation of chromaffin granules, and, upon phosphorylation, induces fusion of the granules' membranes. A similiar phenomenon may occur in the final steps of exocytosis after cytoplasmic annexin translocates to the subplasmalemmal region and is phosphorylated by protein kinase C. However, other studies refute annexin II's involvement in exocytosis, and a conclusive role for annexin II in membrane trafficking remains debated.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610068 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610068 Rev.1
Citations & References
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View product citations for antibody "610068" on CiteAb

Development References (5)

  1. Chasserot-Golaz S, Vitale N, Sagot I, et al. Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells. J Cell Biol. 1996; 133(6):1217-1236. (Biology). View Reference
  2. Choi KS, Fitzpatrick SL, Filipenko NR, et al. Regulation of plasmin-dependent fibrin clot lysis by annexin II heterotetramer. J Biol Chem. 2001; 276(27):25212-25221. (Biology: Immunohistochemistry). View Reference
  3. Hansen MD, Ehrlich JS, Nelson WJ. Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells. J Cell Biol. 2002; 277(47):45371-45376. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Jacovina AT, Zhong F, Khazanova E, Lev E, Deora AB, Hajjar KA. Neuritogenesis and the nerve growth factor-induced differentiation of PC-12 cells requires annexin II-mediated plasmin generation. J Biol Chem. 2001; 276(52):49350-49358. (Biology: Immunofluorescence, Western blot). View Reference
  5. Kwon M, Caplan JF, Filipenko NR, et al. Identification of annexin II heterotetramer as a plasmin reductase. J Biol Chem. 2002; 277(13):10903-10911. (Biology: Flow cytometry). View Reference
View All (5) View Less
610068 Rev. 1

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.