The MHN3-21 monoclonal antibody specifically binds to an extracellular domain of human Notch3. Notch3 is a type 1 transmembrane glycoprotein receptor and member of the Notch family that includes Notch1-Notch4. The Notch3 precursor is cleaved in the Golgi and presents as a cell surface heterodimeric receptor. The Notch3 receptor can bind to several membrane-bound ligands including Jagged1, Jagged2 and Delta1. Upon ligand binding, Notch3 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it can form a transcriptional activator complex with various transcription factors. These multimeric complexes either positively or negatively regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of organ systems such as the hematopoietic, immune, nervous and cardiovascular systems. Notch3 is reportedly expressed by monocytes but not by CD34+ cells and most types of normal lymphocytes. Notch3 has also been implicated in the development of T cell neoplasia. Notch3 is expressed in vascular smooth muscle cells and plays a key role in neural development. Mutations in Notch3 have been identified as the underlying cause of CADASIL, a cerebral autosomal dominant disease that is the most common form of hereditary stroke disorder.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.