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553371testv2.png

Multicolor flow cytometric analysis of CD31 expression on BALB/c mouse bone marrow cells. Bone marrow cells were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD31 antibody (Cat. No. 553371; Right Plot) at 0.25 µg/test followed by FITC Streptavidin (Cat. No. 554060). Bivariate pseudocolor density plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

553371Image1.png

Multicolor flow cytometric analysis of CD31 expression on BALB/c mouse bone marrow cells. Bone marrow cells were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD31 antibody (Cat. No. 553371; Right Plot) at 0.25 µg/test followed by FITC Streptavidin (Cat. No. 554060). Bivariate pseudocolor density plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

553371testv2.png 553371Image1.png Blue-Cap-White-Label.jpg clear-clear-white.jpg

Multicolor flow cytometric analysis of CD31 expression on BALB/c mouse bone marrow cells. Bone marrow cells were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD31 antibody (Cat. No. 553371; Right Plot) at 0.25 µg/test followed by FITC Streptavidin (Cat. No. 554060). Bivariate pseudocolor density plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Multicolor flow cytometric analysis of CD31 expression on BALB/c mouse bone marrow cells. Bone marrow cells were stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553089/553090/561878) and either Biotin Rat IgG2a, κ Isotype Control (Cat. No. 553928; Left Plot) or Biotin Rat Anti-Mouse CD31 antibody (Cat. No. 553371; Right Plot) at 0.25 µg/test followed by FITC Streptavidin (Cat. No. 554060). Bivariate pseudocolor density plots show the correlated expression patterns of CD31 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry and data analysis were performed using a BD FACSCanto II™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Pharmingen™
EndoCAM; GPIIA; PECA1; PECAM1; Platelet endothelial cell adhesion molecule
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG2a, κ
129/Sv mouse-derived endothelioma cell line tEnd.1
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Reported), Immunohistochemistry-paraffin (Not Recommended)
0.5 mg/ml
AB_394817
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Recommended Assay Procedures

For IHC, we recommend the use of purified MEC13.3 mAb in our special formulation for immunohistochemistry, Cat. No. 550274.

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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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553371 Rev. 13
Antibody Details
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MEC 13.3

The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is also expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31.

553371 Rev. 13
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
553371 Rev.13
Citations & References
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View product citations for antibody "553371" on CiteAb

Development References (14)

  1. Baldwin HS, Shen HM, Yan HC, et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development. 1994; 120(9):2539-2953. (Biology). View Reference
  2. Christofidou-Solomidou M, Nakada MT, Williams J, Muller WA, DeLisser HM. Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol. 1997; 158(10):4872-4878. (Biology). View Reference
  3. DeLisser HM, Christofidou-Solomidou M, Strieter RM, et al. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol. 1997; 151(3):671-677. (Biology). View Reference
  4. DeLisser HM, Newman PJ, Albelda SM. Molecular and functional aspects of PECAM-1/CD31. Immunol Today. 1994; 15(10):490-495. (Biology). View Reference
  5. Duncan GS, Andrew DP, Takimoto H, et al. Genetic evidence for functional redundancy of Platelet/Endothelial cell adhesion molecule-1 (PECAM-1): CD31-deficient mice reveal PECAM-1-dependent and PECAM-1-independent functions. J Immunol. 1999; 162(5):3022-3030. (Biology). View Reference
  6. Famiglietti J, Sun J, DeLisser HM, Albelda SM. Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity. J Cell Biol. 1997; 138(6):1425-1435. (Biology). View Reference
  7. Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998; 330(3):1129-1135. (Biology). View Reference
  8. Ling V, Luxenberg D, Wang J, et al. Structural identification of the hematopoietic progenitor antigen ER-MP12 as the vascular endothelial adhesion molecule PECAM-1 (CD31). Eur J Immunol. 1997; 27(2):509-514. (Biology). View Reference
  9. Piali L, Hammel P, Uherek C, et al. CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium. J Cell Biol. 1995; 130(2):451-460. (Biology). View Reference
  10. Rosenblum WI, Murata S, Nelson GH, Werner PK, Ranken R, Harmon RC. Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles. Am J Pathol. 1994; 145(1):33-36. (Biology). View Reference
  11. Suri C, Jones PF, Patan S, et al. Requisite role of angiopoietin-1, a ligand for the TIE2 receptor, during embryonic angiogenesis. Cell. 1996; 87(7):1171-1180. (Biology). View Reference
  12. Tang Q, Hendricks RL. Interferon gamma regulates platelet endothelial cell adhesion molecule 1 expression and neutrophil infiltration into herpes simplex virus-infected mouse corneas. J Exp Med. 1996; 184(4):1435-1447. (Clone-specific: Immunohistochemistry). View Reference
  13. Vanzulli S, Gazzaniga S, Braidot MF, et al. Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors. Biocell. 1997; 21(1):39-46. (Biology). View Reference
  14. Vecchi A, Garlanda C, Lampugnani MG, et al. Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Their application in the identification of adult and embryonic endothelium. Eur J Cell Biol. 1994; 63(2):247-254. (Immunogen). View Reference
View All (14) View Less
553371 Rev. 13

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