Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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TIAR is an RNA-binding protein related to TIA-1. Both proteins are candidate effectors of apoptotic cell death and consist of three N-terminal RNA-recognition motifs (RRM) and a C-terminal protein-interaction domain (PID). Unlike TIA-1, which is localized in the granules of cytotoxic lymphocytes, TIAR is predominantly found in the nucleus of many different cell types. Two related isoforms (42 kDa and 50 kDa) of TIAR have been identified. It has been demonstrated that TIAR can trigger DNA fragmentation in permeabilized thymocytes, suggesting that it may be an effector of apoptotic cell death. During Fas-mediated apoptosis, TIAR shows a rapid translocation from the nucleus to the cytoplasm. This redistribution of TIAR appears to be a specific event and possibly a general feature of the apoptotic program.
Development References (2)
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Kawakami A, Tian Q, Duan X, Streuli M, Schlossman SF, Anderson P. Identification and functional characterization of a TIA-1-related nucleolysin. Proc Natl Acad Sci U S A. 1992; 89(18):8681-8685. (Biology). View Reference
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Taupin JL, Tian Q, Kedersha N, Robertson M, Anderson P. The RNA-binding protein TIAR is translocated from the nucleus to the cytoplasm during Fas-mediated apoptotic cell death. Proc Natl Acad Sci U S A. 1995; 92(5):1629-1633. (Biology). View Reference
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