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Purified Mouse Anti-PKCα
Purified Mouse Anti-PKCα

Western blot analysis of PKCα on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-PKCα antibody.

Purified Mouse Anti-PKCα

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Western blot analysis of PKCα on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-PKCα antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Product Details
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BD Transduction Laboratories™
Protein Kinase C α, PKC-α, PKC-A, PKCA, PRKCA
Rat (QC Testing), Human, Mouse, Dog, Chicken, Frog (Tested in Development)
Mouse IgG2b, κ
Human PKCα aa. 270-427 Recombinant Protein
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry, Immunoprecipitation (Tested During Development)
82 kDa
250 µg/ml
AB_397514
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610108 Rev. 1
Antibody Details
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3/PKCα

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion.  At least eleven isozymes have been described.  These proteins are products of multiple genes and alternative splicing.  Conventional PKC (cPKC) subfamily members (α, β, and γ isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V).  The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester.  However, the the C2-like domains of novel PKC (nPKC) subfamily members (δ, ε, η, and θ isoforms) are Ca2+-independent.  The atypical PKC (aPKC) subfamily members (ζ, ι, and λ isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members.  The C-terminal region of PKC contains the catalytic domain.  The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors.  PKCα regulates a wide variety of functions such as cellular growth, apoptosis, cardiomyocyte function, and brain cognitive functions.

The 3/PKCα monoclonal antibody recognizes PKCα, regardless of phosphorylation status, and has been reported to crossreact with PKCβ.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610108 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610108 Rev.1
Citations & References
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Development References (5)

  1. Nishizuka Y. The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988; 334(6184):661-665. (Biology). View Reference
  2. Powner DJ, Hodgkin MN, Wakelam MJ. Antigen-stimulated activation of phospholipase D1b by Rac1, ARF6, and PKCalpha in RBL-2H3 cells. Mol Biol Cell. 2002; 13(4):1252-1262. (Biology: Immunofluorescence, Western blot). View Reference
  3. Smart EJ, Ying YS, Anderson RG. Hormonal regulation of caveolae internalization. J Cell Biol. 1995; 131(4):929-938. (Biology: Immunohistochemistry). View Reference
  4. Wagner LM, Fowler VM, Takemoto DJ. The interaction and phosphorylation of tropomodulin by protein kinase Calpha in N/N 1003A lens epithelial cells. Mol Vis. 2002; 8:394-406. (Biology: Immunoprecipitation, Western blot). View Reference
  5. Zhang XA, Bontrager AL, Hemler ME.. Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins. J Biol Chem. 2001; 276(27):25005-25013. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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610108 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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