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      Purified Mouse Anti-EphA4/Sek
      Product Details
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      BD Transduction Laboratories™
      Rat (QC Testing), Human, Mouse, Dog, Chicken (Tested in Development)
      Mouse IgG1
      Mouse Sek aa. 279-472
      Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
      110-130 kDa
      250 µg/ml
      AB_397843
      Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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      610471 Rev. 1
      Antibody Details
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      35/EphA4

      EphA4/Sek is a glycosylated protein tyrosine kinase of 986 amino acids and an apparent 110-130kDa molecular weight (molecular weight depends on glycocylation level of protein). EphA4 belongs to the Eph subfamily of receptor tyrosine kinases and like many others, it has an amino-terminal ligand-binding domain exposed to the extracellar matrix. EphA4 is predominantly expressed during development (this is common in the Eph family) and because of its mRNA localization, it has been proposed that it may play a role during segmentation of the hindbrain and mesoderm. Tyrosine 602 is a major autophosphorylation site of EphA4 and appears to be the docking site for the intracellular tyrosine kinase fyn.

      610471 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      610471 Rev.1
      Citations & References
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      Development References (3)

      1. Becker N, Gilardi-Hebenstreit P, Seitanidou T, Wilkinson D, Charnay P. Characterisation of the Sek-1 receptor tyrosine kinase. FEBS Lett. 1995; 368(2):353-357. (Biology). View Reference
      2. Ellis C, Kasmi F, Ganju P, Walls E, Panayotou G, Reith AD. A juxtamembrane autophosphorylation site in the Eph family receptor tyrosine kinase, Sek, mediates high affinity interaction with p59fyn. Oncogene. 1996; 12(8):1727-1736. (Biology). View Reference
      3. Gilardi-Hebenstreit P, Nieto MA, Frain M. An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain. Oncogene. 1992; 7(12):2499-2506. (Biology). View Reference
      610471 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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