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C93420_611377_image1.png

Western blot analysis of CRIK on Rat PC12 cell lysate (ATCC CRL-1721). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-CRIK.

C93420_611376_image2.png

Western blot analysis of CRIK on Human Jurkat cell lysate (ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CRIK (5 min exposure).

C93420_611377_image1.png C93420_611376_image2.png Blue-Cap-White-Label.jpg

Western blot analysis of CRIK on Rat PC12 cell lysate (ATCC CRL-1721). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-CRIK.

Western blot analysis of CRIK on Human Jurkat cell lysate (ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CRIK (5 min exposure).

Product Details
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BD Transduction Laboratories™
Serine/Threonine Protein Kinase 21
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1, κ
Mouse CRIK aa. 1420-1612
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
240 kDa
250 µg/ml
AB_398899
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
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611376 Rev. 2
Antibody Details
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6/CRIK

Citron was identified by its interactions with activated Rho and Rac. It contains a zinc finger (ZF) region, a pleckstrin homology (PH) domain, a citron/MRCK/Gek (CMG) domain, and a coiled-coil domain with a Rho-Rac binding region (RRB). Citron binds to PSD-95 via its C-terminal tSXV motif and the PDZ domain on PSD-95. This association occurs at post synaptic densities where citron-PSD-95 may provide a specific link between the Rho signaling cascade and the synaptic NMDA receptor complex. Citron Rho-interacting kinase (CRIK) and CRIK short kinase (CRIK-SK) are Ser/Thr kinase isoforms of the myotonic dystrophy kinase family. CRIK contains full-length citron, with an N-terminal kinase domain. CRIK-SK is a splice variant that contains only the kinase domain. CRIK co-localizes with actin in keratinocytes and co-expression of CRIK and Rho increases CRIK kinase activity two-fold. In addition, CRIK mutants cause abnormal contraction during cytokinesis. CRIK is expressed highest in testis, but is also present in brain, spleen, lung, and kidney. Thus, CRIK is a Ser/Thr kinase that functions downstream of Rho during events such as cytokinesis and synaptic transmission.

611376 Rev. 2
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611376 Rev.2
Citations & References
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View product citations for antibody "611376" on CiteAb

Development References (4)

  1. Di Cunto F, Calautti E, Hsiao J. Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron. J Biol Chem. 1998; 273(45):29706-29711. (Biology). View Reference
  2. Furuyashiki T, Fujisawa K, Fujita A. Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus. J Neurosci. 1999; 19(1):109-118. (Biology). View Reference
  3. Madaule P, Eda M, Watanabe N. Role of citron kinase as a target of the small GTPase Rho in cytokinesis. Nature. 1998; 394(6692):491-494. (Biology). View Reference
  4. Zhang W, Vazquez L, Apperson M, Kennedy MB. Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. J Neurosci. 1999; 19(1):96-108. (Biology). View Reference
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611376 Rev. 2

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.