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HRP Streptavidin for ELISPOT
Product Details
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ELISPOT (Routinely Tested)
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed

Recommended Assay Procedures

Use with BD ELISPOT pairs

1. Perform a 1:100 dilution of SAv-HRP in Dilution Buffer [10% Fetal Bovine Serum in Phosphate-Buffered Saline (PBS)]: (dissolve 8g NaCl, 0.2 g KCl, 1.44 g Na2HPO4, 0.24 KH2PO4 and bring up with distilled water to 1 liter, adjust pH to 7.2). For optimal results, prepare diluted SAv-HRP solution immediately prior to use.

2. Complete the assay, following instructions in the BD ELISPOT Pair Technical Data Sheet.

3. After use, discard any remaining diluted SAv-HRP solution.

Warning:  Streptavidin-HRP (component 51-9000209) contains 0.004% of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Avoid breathing dust/fume/gas/mist/vapours/spray.

Wear protective gloves/protective clothing/eye protection/face protection.

Contaminated work clothing should not be allowed out of the workplace.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. ProClin is a trademark of Rohm and Haas Company.
  4. Hazardous Ingredient: ProClin™ 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water.
  5. Please refer to to access safety data sheets (SDS).
  6. Please refer to for technical protocols.
557630 Rev. 4
Antibody Details
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Streptavidin-HRP is recommended for use with BD ELISPOT Pairs.

557630 Rev. 4
Format Details
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Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
557630 Rev.4
Citations & References
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No Citations Found

No Citations are available for this Product

557630 Rev. 4

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.