Description
Monokine induced by gamma interferon (MIG) is a recently described chemokine of the CXC subfamily which is one of the collection of proteins encoded by cytokine responsive genes. MIG is inducible in macrophages, hepatocytes, and endothelial cells. The synthesis of MIG is specifically induced by IFN-γ, but not by IFN-α or bacterial lipopolysaccharides. The full-length secreted protein is predicted to have 103 amino acids and a MW of 11,725 daltons. Recombinant human MIG has been reported to induce transient elevation of [Ca2+]i in human tumor infiltrating T lymphocytes (TIL) and in cultured, activated human peripheral blood-derived lymphocytes, but not in human neutrophils, monocytes, or EBV-transformed B lymphoblastoid cell lines.
Formulation and Purity: Recombinant human MIG is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human MIG protein, as measured in a chromogenic LAL assay.
Recombinant human MIG is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 10% glycerol and 1mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
Preparation And Storage
Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 – 1 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 -1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 -10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
Recommended Assay Procedures
ELISA Standard: Recombinant human MIG is useful as a quantitative standard for determining recombinant human MIG protein levels in an MIG specific sandwich ELISA with the purified B8-11 antibody (Cat. No. 555038) as a capture antibody and the biotinylated B8-6 (Cat. No. 555037) as the detection antibody. To obtain linear standard curves, doubling dilutions of this recombinant human MIG standard from ~2,000 to 15 pg/ml should be included in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human MIG in serum or plasma the BD OptEIA™ Human MIG ELISA Set (Cat. No. 550998) is specially formulated and recommended.
Carrier proteins should be pre-screened for possible effects in appropriate experimental system. Carrier proteins may affect experimental results
due to toxicity, high endotoxin levels or possible blocking activity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Development References (5)
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Farber JM. A macrophage mRNA selectively induced by gamma-interferon encodes a member of the platelet factor 4 family of cytokines. Proc Natl Acad Sci U S A. 1990; 87(14):5238-5242. (Biology). View Reference
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Farber JM. HuMig: a new human member of the chemokine family of cytokines. Biochem Biophys Res Commun. 1993; 192(1):223-230. (Biology). View Reference
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Liao F, Rabin RL, Yannelli JR, Koniaris LG, Vanguri P, Farber JM. Human Mig chemokine: biochemical and functional characterization. J Exp Med. 1995; 182(5):1301-1314. (Biology). View Reference
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Loetscher M, Gerber B, Loetscher P, et al. Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes. J Exp Med. 1996; 184(3):963-969. (Biology). View Reference
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Loetscher M, Loetscher P, Brass N, Meese E, Moser B. Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localization. Eur J Immunol. 1996; 28(11):3696-3705. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
