Monokine induced by gamma interferon (MIG) is a recently described chemokine of the CXC subfamily which is one of the collection of proteins encoded by cytokine responsive genes. MIG is inducible in macrophages, hepatocytes, and endothelial cells. The synthesis of MIG is specifically induced by IFN-γ, but not by IFN-α or bacterial lipopolysaccharides. The full-length secreted protein is predicted to have 103 amino acids and a MW of 11,725 daltons. Recombinant human MIG has been reported to induce transient elevation of [Ca2+]i in human tumor infiltrating T lymphocytes (TIL) and in cultured, activated human peripheral blood-derived lymphocytes, but not in human neutrophils, monocytes, or EBV-transformed B lymphoblastoid cell lines.
Formulation and Purity: Recombinant human MIG is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human MIG protein, as measured in a chromogenic LAL assay.
Recombinant human MIG is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 10% glycerol and 1mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.