ELISA Capture: The purified monoclonal antibody 4D5/A7/C5 is useful as a capture antibody in a sandwich ELISA for measuring human IP-10 protein levels. Purified monoclonal antibody 4D5/A7/C5 can be paired with the biotinylated monoclonal antibody 6D4/D6/G2 (Cat. No. 555048) with recombinant human IP-10 (Cat. No. 551130) as the standard. Purified monoclonal antibody 4D5/A7/C5 should be titrated to determine its optimal concentration for ELISA capture (0.5-2.0 µg/ml). To obtain linear standard curves, doubling dilutions of human IP-10 ranging from ~2500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols sections or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, w ww.bdbiosciences.com.
Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines or chemokines tested (human IL-1α, IL-1αβ, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, lymphotactin, MIG, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TGF, TNF, LT-β and mouse Crg-2).
Note 2: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring IP-10 in serum or plasma the BD OptEIA™ Human IP-10 ELISA Set (Cat. No. 550926 ) is specially formulated and recommended.