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      Purified Mouse Anti-Human IgM
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      ELISA (Routinely Tested)
      0.5 mg/ml
      AB_396178
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      This reagent is effective for sandwich human IgM ELISA. mAb JDC-15 is optimal as the capture mAb with clone G20-127 (Cat. No. 555781) as the detecting mAb.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      555856 Rev. 6
      Antibody Details
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      JDC-15

      Reacts specifically with human immunoglobulin M. It does not react with other heavy chain isotypes. This antibody does not stimulate B cells.

      555856 Rev. 6
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      555856 Rev.6
      Citations & References
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      Development References (1)

      1. Zola H, Macardle PJ, Flego L, Webster J. The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry. Dis Markers. 1991; 9(2):103-118. (Biology). View Reference
      555856 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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