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Purified Mouse Anti-Human Eotaxin
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Synthetic human eotaxin
ELISA Capture (Routinely Tested), Western blot (Reported)
0.5 mg/ml
AB_395661
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: The purified monoclonal antibody 3C7 (Cat. No. 555035) is useful as a capture antibody in a sandwich ELISA for measuring human eotaxin protein levels. Purified monoclonal antibody 3C7 can be paired with the biotinylated monoclonal antibody 10C11 (Cat. No. 555060) with recombinant human eotaxin (Cat. No. 555102) as the standard. Purified monoclonal antibody 3C7 should be titrated to determine its optimal concentration for ELISA capture (0.5 - 2.0 µg/ml). To obtain linear standard curves, doubling dilutions of recombinant human eotaxin (Cat. No. 555102) ranging from ~1,000 to 20 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit our web site, www.bdbiosciences.com , and go to the protocols section or the chapter on ELISA in the Immune Function Handbook.

Note 1: This ELISA pair shows no cross-reactivity with any of the chemokines tested (human MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β; mouse Eotaxin, MCP-1; and rat MCP-1).

Note 2: This ELISA pair is recommended primarily for measuring human eotaxin from experimental cell culture systems. For assay of eotaxin from serum or plasma, the specially formulated BD OptEIA™ Human Eotaxin ELISA Set, Cat. No. 555175 is recommended.

WB: The purified 10C11 antibody (Cat. No. 555035) has been reported to be useful for Western Blotting. Please note that this application is not routinely tested at BD Biosciences Pharmingen.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555035 Rev. 1
Antibody Details
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3C7.LS42

The monoclonal antibody 3C7 reacts with human CC chemokine eotaxin. The immunogen used to generate the monoclonal antibody 3C7 was synthetic human eotaxin.

555035 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555035 Rev.1
Citations & References
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Development References (1)

  1. Ponath PD, Qin S, Ringler DJ, et al. Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest. 1996; 97(3):604-612. (Clone-specific). View Reference
555035 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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