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HRP Anti-Human IgG
Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
ELISA (Routinely Tested)
AB_396123
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

Recommended Assay Procedures

Investigators are encouraged to titrate, with a suggested starting dilution of 1:1000.  For sandwich ELISA of human IgG4, HRP-conjugated G18-145 is optimal as detecting mAb with purified JDC-14 (Cat. No. 555878) or G17-4 (Cat. No. 555881) as capture mAb.

Warning:  HRP Mouse Anti-Human IgG contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. ProClin is a trademark of Rohm and Haas Company.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555788 Rev. 13
Antibody Details
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G18-145

IgG is an important component of the humoral immune response, helping to control infection.  IgG is produced by plasma B-cells and may be found in extracellular fluids, such as blood, lymph, peritoneal, and cerebrospinal fluids.  IgG monomers consist of two light and two heavy chains containing two antigen binding sites.  There are four IgG subclasses found in human, mouse and rat species, which include IgG1, IgG2, IgG3 and IgG4.  The G18-145 monoclonal antibody specifically binds to the heavy chain of human immunoglobulin G subclasses: IgG1, IgG2, IgG3 and IgG4.  The G18-145 antibody has been reported not to react with the heavy chains of other human immunoglobulin isotypes.

555788 Rev. 13
Format Details
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HRP
Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
HRP
555788 Rev.13
Citations & References
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Development References (1)

  1. Zola H, Macardle PJ, Flego L, Webster J. The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry. Dis Markers. 1991; 9(2):103-118. (Biology). View Reference
555788 Rev. 13

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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