Preparation And Storage
Recommended Assay Procedures
ELISA Detection: Biotin Rat Anti-Mouse IL-5 antibody (Cat. No. 554397) is useful as a detection antibody for a sandwich ELISA measuring mouse IL-5 protein levels. Biotin Rat Anti-Mouse IL-5 antibody can be paired with the Purified Rat Anti-Mouse/Anti-Human IL-5 antibody (Cat. No. 554393) as the capture antibody, with Recombinant Mouse IL-5 (Cat. No. 554581) as the standard. This biotinylated detection antibody should be titrated in the range of 0.5 to 2.0 µg/ml to determine its optimal concentration for ELISA. To obtain linear standard curves, doubling dilutions of mouse IL-5 ranging from ~15 to 2000 pg/ml are recommended for inclusion in each ELISA plate.
For specific methodology, please visit our web site, http://www.bdbiosciences.com/us/s/resources, and go to the protocols section under "ELISA and ELISPOT".
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum or plasma samples. For measuring Mouse IL-5 in serum or plasma our Mouse IL-5 ELISA Set (Cat. No. 555236) is specially formulated and recommended.
Note: This ELISA pair shows no cross-reactivity with any of the following cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The TRFK4 antibody reacts with mouse interleukin-5 (IL-5). The immunogen used to generate the TRFK4 hybridoma was a semi-purified T cell clone supernatant. This is a neutralizing antibody.
Development References (4)
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Abrams J. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. Curr Protoc Immunol. 2001; 1:6.20-6.21. (Clone-specific: ELISA). View Reference
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific: ELISA). View Reference
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Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Clone-specific: ELISA). View Reference
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Schumacher JH, O'Garra A, Shrader B, et al. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J Immunol. 1988; 141(5):1576-1581. (Clone-specific: ELISA). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.