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Biotin Rat Anti-Mouse C3a
Biotin Rat Anti-Mouse C3a

Representative mouse C3a standard curve. The curve was generated by a sandwich ELISA using Purified Rat Anti-Mouse C3a antibody (Cat. No. 558250) as capture antibody, doubling dilutions of purified mouse C3a protein (Cat. No. 558618) as standard, and Biotin Rat Anti-Mouse C3a antibody (Cat. No. 558251) as detection antibody.  The standard curve is displayed as the concentration of purified mouse C3a in ng/ml versus the microwell absorbance.

Representative mouse C3a standard curve. The curve was generated by a sandwich ELISA using Purified Rat Anti-Mouse C3a antibody (Cat. No. 558250) as capture antibody, doubling dilutions of purified mouse C3a protein (Cat. No. 558618) as standard, and Biotin Rat Anti-Mouse C3a antibody (Cat. No. 558251) as detection antibody.  The standard curve is displayed as the concentration of purified mouse C3a in ng/ml versus the microwell absorbance.

Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG1, κ
ELISA Detection (Routinely Tested)
0.5 mg/ml
AB_2195321
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Biotin Rat Anti-Mouse C3a antibody (Cat. No. 558251) is useful as a detector antibody for a sandwich ELISA that measures mouse C3a protein levels. Purified Rat Anti-Mouse C3a antibody (Cat. No. 558250) can be used as the capture antibody and paired with Biotin Rat Anti-Mouse C3a antibody as the detection antibody, using purified mouse C3a protein as standard (Cat. No. 558618). Purified Rat Anti-Mouse C3a antibody should be titrated between 0.25-2 µg/ml to determine its optimal ELISA detection concentration. To obtain linear standard curves, doubling dilutions of purified mouse C3a protein ranging from 781 pg/ml to 50 ng/ml are recommended for inclusion in each ELISA plate. Addition of FUT-175 (Futhan) to plasma samples at the time of sample collection provides additional protection from ex-vivo activation, and therefore ensures more accurate measurements that reflect the circulating levels of complement activation products. For specific methodology measuring the levels of cytokines, chemokines, and inflammatory mediators and their receptors, see the protocols under "ELISA and ELISPOT" at our website: http://www.bdbiosciences.com/us/s/resources.

For this ELISA antibody pair, no cross-reactivity has been observed with the following: recombinant mouse C5a, native human C3a, C4a and C5a, and rat C5a. This ELISA antibody pair shows a limited cross-reactivity with rat C3a.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
558251 Rev. 5
Antibody Details
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I87-419

The I87-419 antibody reacts with the mouse C3a protein. Anaphylatoxin C3a is a bioactive cleavage product released from plasma component C3 during complement activation and is involved in mediation of a variety of cellular immune responses, as well as being a potent pro-inflammatory agent.  The release of this cleavage product is a reliable indicator of in vivo or in vitro complement activation.  

558251 Rev. 5
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
558251 Rev.5
Citations & References
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Development References (4)

  1. Ember J.A, Jagels M.A., Hugli T.E. Characterization of complement anaphylatoxins and their biologic responses. In: Volenakis JE and Frank MM, ed. The human complement system in health and disease. New York: Marcel Dekker Inc; 1998:241-284.
  2. Hugli T.E. Structure and function of C3a anaphylatoxin . Curr Top Microbiol Immunol. 1990; 153:181-208. (Biology).
  3. Hugli T.E. Structure and function of the anaphylatoxins . Springer Semin Immunopathol. 1984; 7:193-219. (Biology).
  4. Volanakis J.E. Overview of the complement system . In: Volenakis JE and Frank MM, ed. The human complement system in health and disease. New York: Marcel Dekker Inc; 1998:9-32.
View All (4) View Less
558251 Rev. 5

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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