ELISA Detection: The biotinylated B8-6 antibody (Cat. No. 555037) is useful as a detection antibody in a sandwich ELISA for measuring human MIG protein levels. The biotinylated B8-6 antibody can be paired with the purified monoclonal antibody B8-11 (Cat. No. 555038) with recombinant human MIG (Cat. No. 554636) as the standard. The biotinylated B8-6 antibody should be titrated to determine optimal concentration for ELISA detection (2.0 - 6.0 µg/ml). To obtain linear standard curves, doubling dilutions of human MIG ranging from ~2,500 to 39 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.
This ELISA pair shows no cross-reactivity with any of the cytokines tested ( human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL- 11, IL-13, IL-15, IL-16, eotaxin, G-CSF, GM-CSF, GROα, GROβ, GROγ, IFN-γ, IP-10, lymphotactin, MCP-1, MCP-2, MCP-3, MCP-4, MIP-1α, MIP-1β, NAP-2, PF-4, RANTES, TNF, LT-β).
This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assaying serum samples. The OptEIA™ Human MIG ELISA Set (Cat. No. 550998) is specially-formulated for serum cytokine measurement.