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Biotin Mouse Anti-Human CD126
Product Details
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BD Pharmingen™
IL-6 Receptor α Chain
Human (QC Testing)
Mouse IgG1, κ
Mixture of U266, XG-1and BWD41 cells
ELISA Detection (Routinely Tested)
0.5 mg/ml
AB_2127927
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Detection: The Biotinylated M182 antibody (Cat. No. 552503) is useful as a detection antibody for a sandwich ELISA measuring human IL-6Ra protein levels. Biotinylated M182 antibody can be paired with the purified M5 anti-human IL-6Ra (Cat. No. 551462) as the capture antibody, with recombinant soluble IL-6Ra (please inquire) as the standard. Biotin M182 antibody should be titrated between 0.5-2 µg/ml to determine its optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of recombinant human soluble IL-6Ra, ranging from 500 to 2 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology, see BD Pharmingen's Immune Function Handbook, which is posted on our website at www. BDBiosciences.com.

Note: This ELISA antibody pair shows no cross-reactivity with the following recombinant human cytokines:

CD40, CD40L, CD14, IL-1Ra, IL-1R1, IL-1RII, IL-1α, IL-1ß, IL-2, IL-3, IL-4, sIL-4R, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, TNF-α, TNF-ß, sTNFRI, sTNFRII, IFNγ, TRAIL, GM-CSF, TGFß, Trx.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
552503 Rev. 1
Antibody Details
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M182

The M182 antibody reacts with the a subunit of the human IL-6 Receptor (IL-6Rα, aka, CD126). The human IL-6Rα is an 80 kDa type I transmembrane glycoprotein, also known as B cell stimulatory factor-2 (BSF-2) receptor and IL-6 receptor. The IL-6Rα subunit associates with the 130-160 kDa gp130 subunit (IL-6 receptor ß chain, CD130), that is shared with the receptors for Leukemia Inhibitory Factor (LIF), Ciliary Neurotropic Factor (CNTF), Oncostatin M (OSM), IL-11, Cardiotropin 1 (CT-1) and possibly Neurotrophin-1/B Cell-Stimulating Factor 3 (NNT-1/BSF-3). The IL-6Rα chain binds IL-6 with low affinity, however the association with CD130 stabilizes the IL-6/IL-6Rα complex resulting in the formation of a high affinity complex. The IL-6R ß chain mediates signal transduction. IL-6Rα's are expressed at high levels by activated and EBV-transformed B cells, plasma cells and myeloma cells and at lower levels by most leucocytes, epithelial cells, fibroblasts, hepatocytes and neural cells. IL-6Rα exists in soluble form in human serum. The serum levels of soluble IL-6Raappear to elevate in pathological situations such as multiple myeloma, Grave's disease, juvenile chronic arthritis and HIV.10-13 The immunogen used to generate the M182 hybridoma was a mixture of U266, XG-1 (human myeloma cell line expressing membrane IL-6R) and BWD41 cells (murine thymoma cell line transfected with cDNA encoding the extracellular part of IL-6Rα).

552503 Rev. 1
Format Details
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Biotin
Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
Biotin
552503 Rev.1
Citations & References
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Development References (16)

  1. Browning JL, Dougas I, Ngam-ek A, et al. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.. J Immunol. 1995; 154(1):33-46. (Biology). View Reference
  2. Gaillard JP, Bataille R, Brailly H, et al. Increased and highly stable levels of functional soluble interleukin-6 receptor in sera of patients with monoclonal gammopathy. Eur J Immunol. 1993 April; 23(4):820-824. (Biology). View Reference
  3. Heinrich PC, Behrmann I, Müller-Newen G, Schaper F, Graeve L. Interleukin-6-type cytokine signalling through the gp130/Jak/STAT pathway. Biochem J. 1998 September; 334(Pt 2):297-314. (Biology). View Reference
  4. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990; 63(6):1149-1157. (Biology). View Reference
  5. Hirano T, Nakajima K, Hibi M. Signaling mechanisms through gp130: a model of the cytokine system. Cytokine Growth Factor Rev. 1997 December; 8(4):241-252. (Biology). View Reference
  6. Honda M, Yamamoto S, Cheng M, et al. Human soluble IL-6 receptor: its detection and enhanced release by HIV infection. J Immunol. 1992 April; 148(7):2175-2180. (Biology). View Reference
  7. Keul R, Heinrich PC, Müller-newen G, Muller K, Woo P. A possible role for soluble IL-6 receptor in the pathogenesis of systemic onset juvenile chronic arthritis. Cytokine. 1998 September; 10(9):729-734. (Biology). View Reference
  8. Liautard J, Gaillard JP, Mani JC, et al. Epitope analysis of human IL-6 receptor gp80 molecule with monoclonal antibodies.. Eur Cytokine Netw. 1994 May-June; 5(3):293-300. (Biology). View Reference
  9. Müller-Newen G, Köhne C, Keul R, et al. Purification and characterization of the soluble interleukin-6 receptor from human plasma and identification of an isoform generated through alternative splicing. Eur J Biochem. 1996 March; 236(3):837-842. (Biology). View Reference
  10. Salvi M, Girasole G, Pedrazzoni M, et al. Increased serum concentrations of interleukin-6 (IL-6) and soluble IL-6 receptor in patients with Graves' disease. J Clin Immunol. 1996 August; 81(8):2976-2979. (Biology). View Reference
  11. Senaldi G, Varnum BC, Sarmiento U, et al. Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family. Proc Natl Acad Sci U S A. 1999 September; 96(20):11458-11463. (Biology). View Reference
  12. Taga T, Hibi M, Hirata Y, et al. Interleukin-6 triggers the association of its receptor with a possible signal transducer, gp130. Cell. 1989 August; 58(3):573-581. (Biology). View Reference
  13. Taga T, Kawanishi Y, Hardy RR, Hirano T, Kishimoto T. Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression. J Exp Med. 1987 October; 166(4):967-981. (Biology). View Reference
  14. Van Snick J. Interleukin-6: an overview. Annu Rev Immunol. 1990; 8:253-278. (Biology). View Reference
  15. Yamasaki K, Taga T, Hirata Y, et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN beta 2) receptor. Science. 1988 August; 241(4867):825-828. (Biology). View Reference
  16. Zola H. Detection of receptors for cytokines and growth factors. Immunologist. 1994; 2:47-50. (Biology).
View All (16) View Less
552503 Rev. 1

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