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Human Soluble CD14 Flex Set

Human Soluble CD14 Flex Set

(RUO)
Human Soluble CD14 Flex Set

Figure 1. Example BD CBA Human Soluble CD14 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using the FCAP Array Software (Cat. No. 641488).

Human Soluble CD14 Flex Set

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Soluble CD14 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Human Soluble CD14 Flex Set

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

*ND = Not Determined. Due to high levels of CD14 in serum and EDTA-plasma samples, recovery information could not be generated.

Human Soluble CD14 Flex Set

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.  

*ND = Not Determined. Due to high levels of CD14 in serum and EDTA-plasma samples, linearity information could not be generated.

Figure 1. Example BD CBA Human Soluble CD14 Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using the FCAP Array Software (Cat. No. 641488).

Reproducibility: The intra-assay and inter-assay reproducibility were determined for the BD CBA Human Soluble CD14 Flex Set by evaluating ten replicates of three different sample levels (intra-assay) and three replicates of three different sample levels from four separate experiments (inter-assay).

Recovery:        Cell culture supernatant and pooled mouse serum were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum samples were diluted 1:4 before the protein was spiked into each. Serum used was commercially available pooled mouse serum.

*ND = Not Determined. Due to high levels of CD14 in serum and EDTA-plasma samples, recovery information could not be generated.

Linearity: Cell culture supernatant and 1:4 diluted pooled mouse serum were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.  

*ND = Not Determined. Due to high levels of CD14 in serum and EDTA-plasma samples, linearity information could not be generated.

Product Details
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BD™ Cytometric Bead Array (CBA)
D7
40-10,000 pg/mL
Human (QC Testing)
RUO
AB_2869341


Description

The BD™ CBA Human Soluble CD14 Flex Set is a bead-based immunoassay capable of measuring human soluble CD14 in cell culture supernatant samples. Human reactivity was determined by testing samples with the BD CBA Human Soluble CD14 Flex Set. The biology and function of CD14 has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).


This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 ml polypropylene tube for reconstitution. When reconstituted in 4.0 ml Assay Diluent, the standard has a protein concentration of 10,000 pg/ml. Discard unused reconstituted standard, do not store or reuse. Store lyophilized standard and other components at 4°C. Protect PE Detection Reagent from prolonged exposure to light.

Recommended Assay Procedures

The BD CBA Human Soluble CD14 Flex Set must be used in conjunction with a BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264, 100 tests, or 558265, 500 tests), a flow cytometer, and the FCAP Array™ Software (Cat. No. 641488). Detailed instructions on the use of this product can be found in the manual for the BD CBA Human Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Human Soluble CD14 Flex Set will be 10,000 pg/ml. An example standard curve is shown in Figure 1.

Due to high levels of sCD14 in serum and plasma samples, a starting dilution of 1:200 is recommended.

The BD CBA Human Soluble CD14 Flex Set should not be used in the same assay well with any non-BD CBA Human Soluble Protein Flex Set or CD Marker Flex Set reagents (such as BD CBA Mouse Soluble Protein or Cell Signaling Flex Sets) nor with any BD CBA Human Soluble Receptor Protein Flex Set reagents. For technical protocols and additional resources, please visit "Bead-Based Immunoassays" at our website: http://www.bdbiosciences.com/us/s/resources.

Performance

Limit of Detection: The theoretical limit of detection is 9.2 pg/ml and was determined by evaluating the estimated result of the average MFI of the negative control (0 pg/ml, n=30) + 2 standard deviations.

Product Notices

  1. ProClin is a trademark of Rohm and Haas Company.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research.
  5. Warning: CBA lyophilized standard contains 0.02% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of:5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).Hazard statement: May cause an allergic skin reaction.Precautionary statements: Contaminated work clothing should not be allowed out of the workplace. Wear protective gloves/eye/face protection. Wear protective clothing. Avoid breathing mist/vapours/spray. If skin irritation or rash occurs: Get medical advice/attention. IF ON SKIN: Wash with plenty of water. Take off contaminated clothing and wash it before reuse. Dispose of contents/container in accordance with local/regional/national/international regulations.
  6. Hazardous Ingredient: ProClin™ 150. Avoid exposure to skin and eyes and ingestion. Wash exposed skin with soap and water. Flush eyes with water.
560418 Rev. 3
Components
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Description Quantity/Size Part Number
Human Soluble CD14 Standard N/A 51-9006139
Human Soluble CD14 PE Detection Reagent N/A 51-9006141
Human Soluble CD14 Capture Bead D7 100 Tests (1 ea) 51-9006143
560418 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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