PE-Cy™7 Mouse IgG2a, κ Isotype Control

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BD Pharmingen™ PE-Cy™7 Mouse IgG2a, κ Isotype Control

Name: PE-Cy™7 Mouse IgG2a, κ Isotype Control
Brand: PE-Cy™7 Mouse IgG2a, κ Isotype Control
SKU: 557907

Clone G155-178 (RUO)

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Catalog No:557907

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100 Tests

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Product Details

Brand: BD Pharmingen™

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: TNP-keyhole limpet hemocyanin

Application: Flow cytometry, Isotype control (Routinely Tested)

Vol. Per Test: 5 µl

RRID: AB_647254

Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Data Sheets

Technical Data Sheet (TDS)

Safety Data Sheet

557907 Rev. 5

Antibody Details

G155-178

The G155-178 clone has an unknown specificity. Trinitrophenol (TNP), the immunogen, is a hapten not expressed on human, mouse, rat or non-human primate cells. In the absence of specific binding, this antibody may bind non-specifically to immunoglobulin Fc receptors. The immunoglobulin secreted by the G155-178 hybridoma was selected as a mouse IgG2a, κ isotype control following screening for low background binding on a variety of mouse and human tissues.

This immunoglobulin is specific for trinitrophenol (TNP), a hapten not expressed on human cells or human cell lines. In the absence of specific binding, this immunoglobulin may bind non-specifically to Fc receptors.

This antibody is routinely tested on peripheral blood by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

557907 Rev. 5

Format Details

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE-Cy7

Excitation Max: 496 nm, 566 nm

Emission Max: 781 nm

557907 Rev.5

Citations & References

557907 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Pharmingen™ PE-Cy™7 Mouse IgG2a, κ Isotype Control

Clone G155-178 (RUO)

Preparation And Storage

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