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      PE Mouse Anti-Human IL-1ra
      Product Details
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      BD FastImmune™
      Human
      Mouse IgG1
      Recombinant human IL-1 receptor antagonist
      Intracellular staining (flow cytometry)
      20 μL
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The PE conjugate is supplied in 1.0 mL of PBS. PBS contains gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340525 Rev. 1
      Antibody Details
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      AS17

      Anti-Hu–IL-1ra, clone AS17, is derived from the fusion of P3X63.Ag8.653 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-1 receptor antagonist.

      Anti-Human Interleukin-1ra (Anti-Hu–IL-1ra) recognizes a 17- to 25-kilodalton (kd) polypeptide.

      340525 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340525 Rev.1
      Citations & References
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      Development References (25)

      1. Arend W, Joslin F, Massoni J. Effects of immune complexes on production by human monocytes of interleukin-1 or an interleukin-1 inhibitor. J Immunol. 1985; 134:3868-3875. (Biology).
      2. Arend WP, Joslin FG, Thompson RC, Hannum CH. An IL-1 inhibitor from human monocytes: production and characterization of biologic properties. J Immunol. 1989; 143:1851-1858. (Biology).
      3. Arend WP, Smith Jr MF, Janson RW, Joslin FG. IL-1 receptor antagonist and IL-1 β production in human monocytes are regulated differently. J Immunol. 1991; 147:1530-1536. (Biology).
      4. Arend WP. Dixon F, ed. Advances in Immunology. 1993:167-227.
      5. Balavoine J, de Rochemonteix B, Williamson K, Seckinger P, Cruchaud A, Dayer J-M. Prostaglandin E2 and collagenase production by fibroblasts and synovial cells is regulated by urine-derived human interleukin-1 and inhibitors. J Clin Invest. 1986; 78:1120-1124. (Biology).
      6. Casini-Raggi V, Kam L, Chong YJT, et al. Mucosal imbalance of IL-1 and IL-1 receptor antagonist in inflammatory bowel disease: A novel mechanism of chronic intestinal inflammation. J Immunol. 1995; 154:2434-2440. (Biology).
      7. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
      8. Dripps DJ, Brandhuber BJ, Thompson RC, Eisenberg SP. Interleukin-1 (IL-1) receptor antagonist binds to the 80-kDa IL-1 receptor but does not initiate IL-1 signal transduction. J Biol Chem. 1991; 266:10331-10336. (Biology).
      9. Eisenberg SP, Evans RJ, Arend WP, et al. Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist. Nature. 1990; 343:341-346. (Biology).
      10. Evans RJ, Bray J, Childs JD, et al. Mapping receptor binding sites in interleukin (IL)-1 receptor antagonist and IL-1 β by site-directed mutagenesis. J Biol Chem. 1995; 270:11477-11483. (Biology).
      11. Feldmann M, Brennan FM, Maini RN. Rheumatoid arthritis. Cell. 1996; 85:307-310. (Biology).
      12. Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Ann Rev Immunol. 1996; 14:397-440. (Biology).
      13. Ferretti M, Casini-Raggi V, Pizarro RR, et al. Neutralization of endogenous IL-1 receptor antagonist exacerbates and prolongs inflammation in rabbit immune colitis. J Clin Invest. 1994; 94:449-453. (Biology).
      14. Fischer E, Van Zee KJ, Marano MA, et al. Interleukin-1 receptor antagonist circulates in experimental inflammation and in human disease. Blood. 1992; 79:2196-2200. (Biology).
      15. Granowitz EV, Clark BD, Mancilla J, Dinarello CA. Interleukin-1 receptor antagonist competitively inhibits the binding of interleukin-1 to the type II interleukin-1 receptor. J Biol Chem. 1991; 266:14147-14150. (Biology).
      16. Hannum CH, Wilcox CJ, Arend WP, et al. Interleukin-1 receptor antagonist activity of a human interleukin-1 inhibitor. Nature. 1990; 343:336-340. (Biology).
      17. Haskill S, Martin G, Van Le L, et al. cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium. Proc Natl Acad Sci USA. 1991; 88:3681-3685. (Biology).
      18. Janson RW, Hance KR, Arend WR. Production of IL-1 receptor antagonist by human in vitro–derived macrophages. J Immunol. 1991; 147:4218-4223. (Biology).
      19. Kennedy MC, Rosenbaum JT, Brown J, et al. Novel production of interleukin-1 receptor antagonist peptides in normal human cornea. J Clin Invest. 1995; 95:82-88. (Biology).
      20. Krzesicki RF, Hatfield CA, Bienkowski MJ, et al. Regulation of expression of IL-1 receptor antagonist protein in human synovial and dermal fibroblasts. J Immunol. 1993; 150:4008-4018. (Biology).
      21. Liao Z, Grimshaw RS, Rosenstreich DL. Identification of a specific interleukin 1 inhibitor in the urine of febrile patients. J Exp Med. 1984; 159:126-136. (Biology).
      22. Mazzei GJ, Seckinger PL, Dayer J-M, Shaw AR. Purification and characterization of a 26-kDa competitive inhibitor of interleukin-1. Eur J Immunol. 1990; 20:683-689. (Biology).
      23. McColl SR, Paquin R, Ménard C, Beaulier AD. Human neutrophils produce high levels of the interleukin 1 receptor antagonist in response to granulocyte/macrophage colony-stimulating factor and tumor necrosis factor α. J Exp Med. 1992; 176:593-598. (Biology).
      24. Ohlsson K, Björk P, Bergenfeldt M, Hageman R, Thompson RC. Interleukin-1 receptor antagonist reduces mortality from endotoxin shock. Nature. 1990; 348:550-552. (Biology).
      25. Poutsiaka DD, Clark BD, Vannier E, Dinarello CA. Production of interleukin-1 receptor antagonist and interleukin- 1 β by peripheral blood mononuclear cells is differentially regulated. Blood. 1991; 78:1275-1281. (Biology).
      View All (25) View Less
      340525 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not For Use in Diagnostic Procedures.

      RUO/GMP—For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

      23-22943-00

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