Skip to main content Skip to navigation
FITC Mouse Anti-Human IL-6
Product Details
Down Arrow Up Arrow


BD FastImmune™
Human
Mouse IgG1
Recombinant human IL-6 receptor antagonist
Intracellular staining (flow cytometry)
1.5 μg/mL
20 μL
3569
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340526 Rev. 1
Antibody Details
Down Arrow Up Arrow
AS12

The Anti-Hu–IL-6 antibody, clone AS12, is derived from the fusion of P3X63.Ag8.653 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-6.

The Anti-Human Interleukin-6 (Anti-Hu–IL-6) antibody recognizes a 21- to 29-kilodalton (kDa) polypeptide.

340526 Rev. 1
Format Details
Down Arrow Up Arrow
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
FITC
Blue 488 nm
494 nm
518 nm
340526 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (24)

  1. Akira S, Taga T, Kishimoto T. Interleukin-6 in biology and medicine. Adv Immunol. 1993; 54:1-79. (Biology).
  2. Barlogie B, Epstein J, Selvanayagam P, Alexanian R. Plasma cell myeloma: New biological insights and advances in therapy. Blood. 1989; 73:865-879. (Biology).
  3. Birx DL, Redfield RR, Tencer K, et al. Induction of interleukin-6 during human immunodeficiency virus infection. Blood. 1990; 76:2303-2310. (Biology).
  4. Breen EC, Rezai AR, Nakajuma K, et al. Infection with HIV is associated with elevated IL-6 levels and production. J Immunol. 1990; 144:480-484. (Biology).
  5. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Dokter WHA, Koopmans SB, Vellenga E. Effects of IL-10 and IL-4 on LPS-induced transcription factors (AP-1, NF-IL6 and NF-κB) which are involved in IL-6 regulation. Leukemia. 1996; 10:1308-1316. (Biology).
  8. Feldmann M, Brennan FM, Maini RN. Rheumatoid arthritis. Cell. 1996; 85:307-310. (Biology).
  9. Feldmann M, Brennan FM, Maini RN. Role of cytokines in rheumatoid arthritis. Ann Rev Immunol. 1996; 14:397-440. (Biology).
  10. Gauldie J, Richards C, Harnish D, Lansdorp P, Baumann H. Interferon β 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc Natl Acad Sci USA. 1987; 84:7251-7256. (Biology).
  11. Hibi M, Murakami M, Saito M, Hirano T, Taga T, Kishimoto T. Molecular cloning and expression of an IL-6 signal transducer, gp130. Cell. 1990; 63(6):1149-1157. (Biology). View Reference
  12. Iwakura Y, Tosu M, Yoshida E, et al. Induction of inflammatory arthropathy resembling rheumatoid arthritis in mice transgenic for HTLV-1. Science. 1991; 253:1026-1028. (Biology).
  13. Kishimoto T, Akira S, Taga T. Interleukin-6 and its receptor: a paradigm for cytokines. Science. 1992; 258(5082):593-597. (Biology). View Reference
  14. Kishimoto T. The biology of interleukin-6. Blood. 1989; 74:43840. (Biology).
  15. Kohase M, May LT, Tamm I, Vilcek J, Sehgal PB. A cytokine network in human diploid fibroblasts: Interactions of β -interferons, tumor necrosis factor, platelet-derived growth factor, and interleukin-1. Mol Cell Biol. 1987; 7:273-280. (Biology).
  16. Koike K, Nakahata T, Takagi M, et al. Synergism of BSF-2/interleukin 6 and interleukin 3 on development of multipotential hemopoietic progenitors in serum-free culture. J Exp Med. 1988; 168:879-890. (Biology).
  17. Libermann TA, Baltimore D. Activation of interleukin-6 gene expression through the NF-κB transcription factor. Mol Cell Biol. 1990; 10:2327-2334. (Biology).
  18. Lotz M, Jirik F, Kabouridis P, et al. B cell stimulating factor 2/interleukin 6 is a costimulant for human thymocytes and T lymphocytes. J Exp Med. 1988; 167:1253-1258. (Biology).
  19. May LT, Ghrayeb J, Santhanam U, et al. Synthesis and secretion of multiple forms of β2-interferon/B-cell differentiation factor 2/hepatocyte-stimulating factor by human fibroblasts and monocytes. J Biol Chem. 1988; 263:7760-7765. (Biology).
  20. Muraguchi A, Hirano T, Tang B, et al. The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J Exp Med. 1988; 167:332-344. (Biology).
  21. Muraguchi A, Kishimoto T, Miki Y, et al. T-cell replacing factor–(TRF) induced IgG secretion in a human B blastoid cell line and demonstration of acceptors for TRF. J Immunol. 1981; 127:412-416. (Biology).
  22. Van Damme J, Opdenakker G, Simpson RJ, et al. Identification of the human 26-kD protein, interferon β 2 (IFN- β2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med. 1987; 165:914-919. (Biology).
  23. Van Snick, J. Interleukin 6: an overview. Ann Rev Immunol. 1990; 8:253-278. (Biology).
  24. Zhang Y, Broser M, Rom WN. Activation of the interleukin 6 gene by mycobacterium tuberculosis or lipopolysaccharide is mediated by nuclear factors NF-IL6 and NF-κB. Proc Natl Acad Sci USA. 1994; 91:2225-2229. (Biology).
View All (24) View Less
340526 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not For Use in Diagnostic Procedures.

RUO/GMP—For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

23-22943-00