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FITC Mouse Anti-BrdU
Product Details
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BD™
Mouse IgG1
Iodouridine
Flow cytometry, Intracellular staining (flow cytometry)
25 μg/mL
20 μL
AB_400327
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

The monoclonal antibody is supplied as 50 µg purified immunoglobulin in 2.0 mL (25 µg/mL) of phosphate-buffered saline. The FITC conjugate is supplied as 50 µg in 2.0 mL (25 µg/mL). Buffered saline contains gelatin and 0.1% sodium azide. The vials should be stored at 2º to 8ºC. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

347583 Rev. 1
Antibody Details
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B44

The Anti-BrdU antibody, clone B44, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with spleen cells from BALB/c mice immunized with iodouridine-conjugated ovalbumin.

Bromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated specifically into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single-stranded DNA, free BrdU, or BrdU coupled to a protein carrier. The antibody also reacts with iodouridine. DNase-I cleaves each strand of DNA at random and permits the binding of Anti-BrdU antibody to its antigen (BrdU).

347583 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
347583 Rev.1
Citations & References
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View product citations for antibody "347583" on CiteAb

Development References (7)

  1. Beisker W, Dolbeare F, Gray JW. An improved immunocytochemical procedure for high-sensitivity detection of incorporated bromodeoxyuridine. Cytometry. 1987; 8:235. (Biology).
  2. Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW. Cytochemistry for bromodeoxyuridine/DNA analysis: Stoichiometry and sensitivity. Cytometry. 1985; 6:521-530. (Biology).
  3. Dolbeare F, Gratzner H, Pallavicini MG, Gray JW. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci U S A. 1983; 80(18):5573-5577. (Biology). View Reference
  4. Gratzner HG. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218:474. (Biology).
  5. Gray JW. Monoclonal antibodies against bromodeoxyuridine (special issue). Science. 1985; 6:501-673. (Biology).
  6. Nagashima T, Hoshino T. Rapid detection of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in 9L brain tumor cells in vitro and in situ. Acta Neuropathol (Berl). 1985; 66:12. (Biology).
  7. Pinkel D, Thompson LH, Gray JW, Vanderlaan M. Measurement of sister chromatid exchanges at very low bromodeoxyuridine substitution levels using a monoclonal antibody in Chinese hamster ovary cells. Cancer Res. 1985; 45:5795. (Biology).
View All (7) View Less
347583 Rev. 1

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