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Overview

The BD® OMICS-Guard Sample Preservation Buffer is developed and optimized to address the need for high-quality biological sample preservation over an extended period of time when samples cannot be processed at the same time or location.

 

  • Provides a stress-free, one-step preservation protocol with minimum hands-on time
  • Optimized to preserve cells for a variety of downstream transcriptomic, proteomic and multiomic applications, including RNA-seq, CITE-seq, flow cytometry and qPCR​
  • Protects cell viability and preserves different cell populations in your samples for up to 72 hours at 4 °C
  • Developed and tested across multiple sample types: PBMCs and tissue samples
  • Available in two, easy-to-use formats: 50-mL bottle or 12 x 1-mL vials

 

Download our datasheet to learn more about the BD® OMICS-Guard Sample Preservation Buffer.

 
BD OMICS Guard Sample Preservation Buffer
performance1

One-Step Sample Preservation Workflow

 

 

Single-cell suspension

Tissue

Whole blood

Recommended usage

10,000 to 10 million cells per 1 mL BD® OMICS-Guard SPB

30 to 50 mg of tissue per 20 mL BD® OMICS-Guard SPB

1:1 Ratio of whole blood (with EDTA) and BD® OMICS-Guard SPB

Storage temperature

4 °C 

Storage duration

Up to 72 hours

Buffer handling

BD® OMICS-Guard SPB should be used with aseptic techniques to prevent contamination of the stock buffer. We recommend working under a laminar flow hood

Sample preservation protocol

  1. Collect single cells/dissociated single cells in suspension and spin at 400 x g for 5 min.
  2. Discard supernatant and resuspend the cells in BD® OMICS-Guard SPB as recommended above.
  3. Place cells at 4 °C for up to 72 h.
  4. After storage, spin cells at 800 x g for 5 min and discard the supernatant to remove the BD® OMICS-Guard SPB. No further washing is required.
  5. Resuspend the cells in desired buffer for downstream applications.
  1. Cut tissue into 30–50 mg pieces  and immediately place them into the BD® OMICS-Guard SPB.
  2. Place the preserved tissue at 4 °C for up to 72 h.
  3. After storage, dissociate the tissue by the desired method for your single-cell application.
  4. Resuspend the cells in desired buffer for downstream applications.
  1. Collect whole blood using EDTA as an anti-coagulant and mix with the same volume of BD® OMICS-Guard SPB by inverting 10 times.

    Note:     It may be possible to use other anti-coagulants, though they have not been tested.

  2. Place the whole blood and BD® OMICS-Guard SPB mixture at 4 °C for up to 72 h.
  3. After storage, isolate desired cells.
    • Leukocytes: use a magnetic red blood cell depletion kit to remove the red blood cells and isolate PBMCs and granulocytes.
    • PBMCs: use density gradient separation methods (e.g., STEMCELL SepMate™ PBMC Isolation Tubes).
  4. Spin the isolated cells at 200 x g for 7 min to remove platelets.
  5. Resuspend cells in desired buffer for downstream applications.

Modifications to single-cell capture on the BD Rhapsody™ Single-Cell Analysis System

For cells/tissues/whole blood preserved in BD® OMICS-Guard SPB, ensure that the lysis time in the single-cell capture workflow is at least 5 min (can be longer per the specific assay instruction).

A Novel Cell Preservation Solution

Watch our webinar on how the BD® OMICS-Guard Sample Preservation Buffer is used in the single cell multiomics analysis of tumor samples and showed how it can reveal the immunogenomic mechanisms of immune suppression and cancer immunotherapy.

The speakers also shared their findings on the role of PD-1 expression and metabolic reprogramming in Treg cells and how they affect the response to PD-1 blockade in different tumor types and microenvironments.

 

One-Step Sample Preservation Workflow

 

 Single-Cell SuspensionTissue

Recommended Usage

1 to 10 million cells per 1 mL BD® OMICS-Guard Preservation Buffer

30 to 50 mg of tissue per 20 mL BD® OMICS-Guard Preservation Buffer

Storage Temperature

4 °C

Storage Duration

Up to 72 hours

 
  1. Collect single cells/dissociated single cells in suspension and spin at 400 x g for 5 minutes.

  2. Discard the supernatant and resuspend the cells in the BD® OMICS-Guard Preservation Buffer per the recommended usage listed above
    Note: Cells can be stored in Eppendorf tubes or equivalent.

  3. Place the cells at 4 °C for up to 72 hours.

  4. After storage, spin the cells at 800 x g for 5 minutes and discard the supernatant to remove the BD® OMICS-Guard Preservation Buffer. No further washing is required.

  5. Resuspend the cells in desired buffer for downstream applications.
  1. Section tissues into pieces and immediately put into the BD® OMICS-Guard Preservation Buffer.
    Note: aim for sections that are approximately 50 mg in weight.

  2. Place the tissue at 4 °C for up to 72 hours.

  3. After storage, dissociate the tissue by the desired method for single-cell application, spin the cells at 800 x g for 5 minutes, and discard the supernatant to remove the BD® OMICS-Guard Preservation Buffer. No further washing is required.

  4. Resuspend the cells in desired buffer for downstream applications.

Modifications to single-cell capture on the BD Rhapsody™ Single-Cell Analysis System

For cells/tissues preserved in BD® OMICS-Guard Preservation Buffer, change the lysis conditions in the single-cell capture workflow from 2 minutes to 5 minutes lysis time.

  

Improve Ab-Oligo Signal with the BD® AbSeq Enhancer Kit for BD® OMICS-Guard Buffer-Preserved Samples in CITE-seq

 

If staining with BD® AbSeq Antibody-Oligos after BD® OMICS-Guard Buffer preservation, use the BD® AbSeq Enhancer Kit to reduce nonspecific binding events and enhance the AbSeq signal.

 

Improve Ab-Oligo Signal with the BD AbSeq-Enhancer Kit
apps1

Heatmaps of AbSeq performance with and without the addition of the BD® AbSeq Enhancer Kit. Heatmaps of AbSeq median molecules per cell expression for different cell types are shown when the BD® AbSeq Enhancer Kit is either not added to the protocol (top) or added to the protocol (bottom) when using preserved cells. Nonspecific background signal (top) is eliminated, leading to better signal/noise, with the use of the BD® AbSeq Enhancer Kit (bottom), which closely matches that of the control data (bottom, left plot).

 

Download the BD® OMICS-Guard Sample Preservation Buffer product information sheet to access BD® AbSeq Enhancer Kit staining protocols

Applications

BD® OMICS-Guard Sample Preservation Buffer preserves cell viability, transcriptomic and proteomic profiles, and cell populations in human PBMCs for CITE-seq analyses

A time course CITE-seq analysis was conducted on the BD Rhapsody™ Single-Cell Analysis System with non-preserved (control) human PBMCs (0 h) and BD® OMICS-Guard Buffer-preserved human PBMCs at 24, 48 and 72 h. PBMCs at each time point were stained with a 30-plex BD® AbSeq Ab-Oligo Panel. Cell viability, gene expression, surface protein expression and the cell population in the preserved PBMCs at 24, 48 and 72 h were analyzed and compared to that of the control PBMC sample (n = 2)
OMICS-Guard-Preserved-vs-Nonpreserved-Gene-Expression-Correlation
OMICS-Guard-Preserved-vs-Nonpreserved-Protein-Correlation
Human-PBMC-WTA-Assay-Sensitivity
AbSeq-Sensitivity-in-Major-PBMC-Cells
Human-PBMC-Donor-1
BD® OMICS-Guard Sample Preservation Buffer preserves cell viability, transcriptome and proteome profiles, and cell populations in mouse spleen tissue for CITE-seq analyses

A time course CITE-seq analysis was conducted on the BD Rhapsody™ Single-Cell Analysis System with non-preserved (control) mouse spleen tissues (0 h) and BD® OMICS-Guard Buffer-preserved mouse spleen tissues at 24, 48 and 72 h. Dissociated mouse splenocytes at each time point were stained with a 30-plex BD® AbSeq Ab-Oligo Panel. Cell viability, gene expression, surface protein expression and the cell population in preserved mouse spleen tissues at 24, 48 and 72 h were analyzed and compared to that of control mouse spleen tissues (n = 2).
OMICS-Guard-Gene-Expression-Correlation
OMICS-Guard-Mouse-Spleen-Protein-Expression
Mouse-Splenocyte-WTA-Assay-Sensitivity
Mouse-Splenocyte-AbSeq-Sensitivity-Median-Molecules
Mouse-Spleen-Tissue-Sample-1
BD® OMICS-Guard Sample Preservation Buffer preserves surface protein epitopes and cell populations in human PBMCs for flow cytometry analyses

A time course flow cytometry analysis was conducted using non-preserved (control) human PBMCs (0 h) and BD® OMICS-Guard Buffer-preserved human PBMCs at 24, 48 and 72 h (n = 2). Human PBMCs at each time point were stained with a 13-color fluorescence antibody panel for major immune cell population identification and protein expression analyses and control (0 h) and preserved (24, 48 and 72 h) human PBMCs were compared.
13-plex-Human-Fluorescence-Antibody
omics-guard/applications/slide-3/b/Non-preserved-and-Preserved-Human-PBMCs-Protein-Marker
CD45-Immune-Cell-Population-Frequencies
BD® OMICS-Guard Sample Preservation Buffer preserves protein information in mouse spleen tissue

A time-course flow cytometry analysis was conducted using non-preserved (control) mouse spleen tissues (0 h) and BD® OMICS-Guard Buffer-preserved mouse spleen tissues at 24, 48 and 72 h (n = 2). Dissociated mouse splenocytes at each time point were stained with an 11-color fluorescence antibody panel for major splenic cell population identification and protein expression analyses and control (0 h) and preserved (24, 48 and 72 h) mouse spleen tissues were compared. The protein expression level and frequencies of major cell populations revealed by flow cytometry analyses in mouse splenocytes stayed consistent over the course of 72 hours, indicating the capability of BD® OMICS-Guard Preservation Buffer to preserve surface protein epitopes and cell composition for flow cytometry analyses.
omics-guard/applications/slide-4/a/Mouse-Spleen-Tissue-SPB-Protein-Preservation
Mouse-Spleen-Consistent-Key-Protein-Marker-Expresseion
Mouse-Spleen-CD45-Major-Leucocyte-Cell-Population-Consistent-Frequencies
Stress-free sample preservation with BD® OMICS-Guard Sample Preservation Buffer demonstrated by expression analysis of 84 stress-associated genes in human PBMCs using qPCR

Expression of 84 stress-associated genes were compared between fresh and preserved human PBMC samples using qPCR (n = 2). No significant expression changes of stress-associated genes were observed after 48 h of preservation, indicating a stress-free preservation workflow with BD® OMICS-Guard Preservation Buffer.
Human-PBMC-Stress-Associated-Gene-Expression-Comparison
Donors-1-and-2-qPCR-Analysis

  

My Ha

"OMICS-guard allows me to divide a long procedure of sorting cells and extracting RNA into separate days without compromising the sample quality. Its preservation time of up to 72 hours also facilitates better sample multiplexing in omics applications."

My Ha
PhD
Universiteit Antwerpen
Silbie Sinkunaite

 

"Analyzing transcriptomics in fragile and short-lived cell types, particularly in multi-facility settings, often presents logistical hurdles. This issue is exacerbated when studying granulocytes, like basophils, under different stimulation conditions, where time is of the essence. The "BD OMICS Guard Sample Preservation Buffer" has proven instrumental in preserving samples and extending processing time before the BD Rhapsody System. It also aids in obtaining cleaner proteomic and transcriptomic data, even when working with basophils with low RNA yields."

Sibilė Šinkūnaitė
Research Assistant
Herlev og Gentofte Hospital, Laboratory of Medical Allergology (LMA)
Sabrina Pollastro

"After staining our precious samples with antibodies, we resuspended them in OMICS-Guard. We then sorted B cells into collection tubes containing OMICS Guard prior to loading them into the BD Rhapsody Express system. Cell viability substantially improved compared to the cells kept in staining buffer or EDTA-supplemented staining buffer even when waiting up to 2h before the sorting steps. Overall, we are very satisfied with the performance of OMICS Guard."

Sabrina van der Dussen-Pollastro
PhD
Department of Immunopathology, Sanquin Research, Amsterdam, The Netherlands

     

      

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Alexa Fluor is a trademark of Life Technologies Corporation. CF is a trademark of Biotium, Inc. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.