Skip to main content Skip to navigation
Purified Mouse Anti-RORγt
Purified Mouse Anti-RORγt

Western blot analysis of RORγt expression by mouse thymocytes. Cell lysate was prepared from untreated C57BL/6 mouse thymus (lanes 1-3). The lysate was electrophoresed (SDS-PAGE; approximately 12.5 μg of total cellular protein per lane), transferred to a PVDF membrane and then probed with Purified Mouse Anti-RORγt antibody (Clone O28-835; Cat. No. 562197) at concentrations of 1.25 (lane 1), 0.6 (lane 2), and 0.3 µg/ml (lane 3). Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002) and a chemiluminescent detection system. RORgt is identified as a band of ~56 kDa in the thymus cell lysate.

Western blot analysis of RORγt expression by mouse thymocytes. Cell lysate was prepared from untreated C57BL/6 mouse thymus (lanes 1-3). The lysate was electrophoresed (SDS-PAGE; approximately 12.5 μg of total cellular protein per lane), transferred to a PVDF membrane and then probed with Purified Mouse Anti-RORγt antibody (Clone O28-835; Cat. No. 562197) at concentrations of 1.25 (lane 1), 0.6 (lane 2), and 0.3 µg/ml (lane 3). Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002) and a chemiluminescent detection system. RORgt is identified as a band of ~56 kDa in the thymus cell lysate.

Product Details
Down Arrow Up Arrow


BD Pharmingen™
Rorc; Rorg; RORgamma; Rorγ; RORgt; RORgammat; RORγt; TOR; Thor; Nr1f3; ROR
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG2b, κ
Human RORγt Recombinant Protein
Western blot (Routinely Tested)
56–58 kDa
0.5 mg/ml
AB_10894594
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
562197 Rev. 1
Antibody Details
Down Arrow Up Arrow
O28-835

The O28-835 monoclonal antibody recognizes ROR-gamma (RORγ) and its isoform, RORγt. RORγ (~58 kDa calculated molecular weight) is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. It is expressed in different tissues including skeletal muscle, kidney, liver and adipose tissues. RORγt (~56 kDa calculated molecular weight) is a RORγ isoform that is expressed exclusively by lymphoid cells. It is expressed by various lymphoid cell types including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells and lymphoid tissue inducer (Lti) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches.

562197 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
562197 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (6)

  1. Eberl G, Littman DR. The role of the nuclear hormone receptor RORgt in the development of lymph nodes and Peyer's patches. Immunol Rev. 2003; 195(195):81-90. (Biology). View Reference
  2. Eberl G, Marmon S, Sunshine MJ, Rennert PD, Choi Y, Littman DR. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol. 2004; 5(1):64-73. (Biology). View Reference
  3. Hamada H, Garcia-Hernandez MdlL, Reome JB, et al. Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge. J Immunol. 2009; 182(6):3469-3481. (Biology). View Reference
  4. He YW, Deftos ML, Ojala EW, Bevan MJ. RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells. Immunity. 1998; 9(6):797-806. (Biology). View Reference
  5. Ivanov, II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006; 126(6):1121-1133. (Biology). View Reference
  6. Medvedev A, Yan ZH, Hirose T, Giguere V, Jetten AM. Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element. Gene. 1996; 181(1-2):199-206. (Biology). View Reference
View All (6) View Less
562197 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.