BD Pharmingen™ Purified Mouse Anti-Human IKKβ w/ Control
(RUO)
Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
Western blot analysis of Ikkβ. Lysate from HeLa cells was probed with anti-Ikkβ (clone F18-1875, Cat. No. 51-8120GR) at concentrations of 0.25 (lane 1), 0.125 (lane 2), and 0.06 µg/ml (lane 3). Ikkβ is identified as a band of ~87 kDa.
Product Details
BD Pharmingen™
Human (QC Testing)
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development)
RUO
AB_647275
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Recommended Assay Procedures
Applications include western blot analysis (0.06 - 0.25 µg/ml). Other applications not routinely tested at BD Biosciences Pharmingen include immunoprecipitation (4 µg/sample). Store the antibody at -20°C. HeLa control lysate [50 µg (1 µg/µl)] is provided as a positive control (Cat. No. 51-16516N; store lysate at -20°C). HeLa control lysate (Cat. No. 611449) is sold separately as a ready-to-use western blot control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
HRP Goat Anti-Mouse Ig RUO
Size
1 mL
Cat No.
554002
HeLa Cell Lysate RUO
Size
500 µg
Cat No.
611449
551920 Rev. 7
Components
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Purified Mouse Anti-Human IKKβ | 50 µg (1 ea) | 51-8120GR | N/A |
| HeLa Control Lysate | 50 µg (1 ea) | 51-16516N | N/A |
551920 Rev. 7
Citations & References
Development References (11)
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DiDonato JA, Hayakawa M, Rothwarf DM, Zandi E, Karin M. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB. Nature. 1997; 388(6642):548-554. (Biology). View Reference
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Israël A. The IKK complex: an integrator of all signals that activate NF-kappaB. Trends Cell Biol. 2000; 10(4):129-133. (Biology). View Reference
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Lee FS, Hagler J, Chen ZJ, Maniatis T. Activation of the IkappaB alpha kinase complex by MEKK1, a kinase of the JNK pathway. Cell. 1997; 88(2):213-222. (Biology). View Reference
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Li Q, Van Antwerp D, Mercurio F, Lee KF, Verma IM. Severe liver degeneration in mice lacking the IkappaB kinase 2 gene. Science. 1999; 284(5412):321-325. (Biology). View Reference
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Li ZW, Chu W, Hu Y, et al. The IKKbeta subunit of IkappaB kinase (IKK) is essential for nuclear factor kappaB activation and prevention of apoptosis. J Exp Med. 1999; 189(11):1839-1845. (Biology). View Reference
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Ling L, Cao Z, Goeddel DV. NF-kappaB-inducing kinase activates IKK-alpha by phosphorylation of Ser-176. Proc Natl Acad Sci U S A. 1998; 95(7):3792-3797. (Biology). View Reference
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Malinin NL, Boldin MP, Kovalenko AV, Wallach D. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1. Nature. 1997; 385(6616):540-544. (Biology). View Reference
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Mercurio F, Zhu H, Murray BW, et al. IKK-1 and IKK-2: cytokine-activated IkappaB kinases essential for NF-kappaB activation. Science. 1997; 278(5339):860-866. (Biology). View Reference
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Nakano H, Shindo M, Sakon S, et al. Differential regulation of IkappaB kinase alpha and beta by two upstream kinases, NF-kappaB-inducing kinase and mitogen-activated protein kinase/ERK kinase kinase-1. Proc Natl Acad Sci U S A. 1998; 95(7):3537-3542. (Biology). View Reference
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Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-gamma is an essential regulatory subunit of the IkappaB kinase complex. Nature. 1998; 395(6699):297-300. (Biology). View Reference
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Tanaka M, Fuentes ME, Yamaguchi K, et al. Embryonic lethality, liver degeneration, and impaired NF-kappa B activation in IKK-beta-deficient mice. Immunity. 1999; 10(4):421-429. (Biology). View Reference
551920 Rev. 7
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.