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Western blot analysis of rabaptin-5 on a MCF-7 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the anti- rabaptin-5 antibody.
Immunofluorescence staining of A431 cells.
BD Transduction Laboratories™ Purified Mouse Anti-Rabaptin-5
BD Transduction Laboratories™ Purified Mouse Anti-Rabaptin-5
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
Rabs are small GTP-binding proteins that are localized to specific intracellular vesicles and organelles. They are important for the regulation of vesicular traffic. It is thought that Rabs cycle between GTP- and GDP-bound forms and this cycle is related to their function. Rabaptin-5 is protein that interacts with GTP-Rab5. Although Rabaptin-5 is localized in the cytosol, it is also recruited to the endosomal fraction in a Rab5 and GTP-dependent manner. The removal of Rabaptin-5 from the cytosol substantially impairs GTP and Rab5-dependent endosomal fusion. This indicates that Rabaptin-5 may be an important partner of Rab5 during endocytosis. Rabaptin-5 contains 862 amino acids and both ends of the protein contain coiled-coil domains typically found in vesicular transport proteins.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Shiba Y, Takatsu H, Shin HW, Nakayama K. Gamma-adaptin interacts directly with Rabaptin-5 through its ear domain. J Biochem (Tokyo). 2002; 131(3):327-336. (Biology: Western blot). View Reference
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Stenmark H, Vitale G, Ullrich O, Zerial M. Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion. Cell. 1995; 83(3):423-432. (Biology). View Reference
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Zhu Y, Doray B, Poussu A, Lehto VP, Kornfeld S. Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor. Science. 2001; 292(5522):1716-1718. (Biology: Immunofluorescence, Immunoprecipitation). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.