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Western blot analysis of p130 [Cas] on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- p130 [Cas] antibody.
Immunofluorescence staining of human fibroblasts.
BD Transduction Laboratories™ Purified Mouse Anti-p130 [Cas]
BD Transduction Laboratories™ Purified Mouse Anti-p130 [Cas]
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
p47 [v-crk] is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-crk protein is a fusion product of the viral gag protein and a part of cellular crk that includes SH2 and SH3 domains. v-crk induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130 [Cas]. p130 [Cas] is tightly associated with v-crk via the SH2 domain of v-crk. Tyrosine phosphorylation of p130 [Cas] occurs in conjunction with cellular transformation in cells that express v-src or v-crk. This phosphorylation leads to a change in p130 [Cas] localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130 [Cas] also associates with v-src, it may be a v-src substrate.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (5)
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Derkinderen P, Toutant M, Kadare G, Ledent C, Parmentier M, Girault JA. Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus. J Biol Chem. 2001; 276(41):38289-38296. (Biology: Immunoprecipitation, Western blot). View Reference
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Hermanto U, Zong CS, Li W, Wang LH. RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix. Mol Cell Biol. 2002; 22(7):2345-2365. (Biology: Immunoprecipitation, Western blot). View Reference
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Kook S, Shim SR, Choi SJ, et al . Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells. Mol Biol Cell. 2000; 11(3):929-939. (Biology: Western blot). View Reference
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Nagashima K, Endo A, Ogita H, et al. Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells. Mol Biol Cell. 2002; 13(12):4231-4242. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
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Sakai R, Iwamatsu A, Hirano N, et al. A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner. EMBO J. 1994; 13(16):3748-3756. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.