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Purified Mouse Anti-p130 [Cas]
Purified Mouse Anti-p130 [Cas]
Western blot analysis of p130 [Cas] on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- p130 [Cas] antibody.
Purified Mouse Anti-p130 [Cas]
Immunofluorescence staining of human fibroblasts.
Western blot analysis of p130 [Cas] on a human endothelial cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- p130 [Cas] antibody.
Immunofluorescence staining of human fibroblasts.
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog, Chicken (Tested in Development)
Mouse IgG1
Rat p130 [Cas] aa. 644-819
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Immunoprecipitation (Tested During Development)
130 kDa
250 µg/ml
AB_397666
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610271 Rev. 1
Antibody Details
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21/p130[Cas]

p47 [v-crk] is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-crk protein is a fusion product of the viral gag protein and a part of cellular crk that includes SH2 and SH3 domains. v-crk induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130 [Cas]. p130 [Cas] is tightly associated with v-crk via the SH2 domain of v-crk. Tyrosine phosphorylation of p130 [Cas] occurs in conjunction with cellular transformation in cells that express v-src or v-crk. This phosphorylation leads to a change in p130 [Cas] localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130 [Cas] also associates with v-src, it may be a v-src substrate.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610271 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610271 Rev.1
Citations & References
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View product citations for antibody "610271" on CiteAb

Development References (5)

  1. Derkinderen P, Toutant M, Kadare G, Ledent C, Parmentier M, Girault JA. Dual role of Fyn in the regulation of FAK+6,7 by cannabinoids in hippocampus. J Biol Chem. 2001; 276(41):38289-38296. (Biology: Immunoprecipitation, Western blot). View Reference
  2. Hermanto U, Zong CS, Li W, Wang LH. RACK1, an insulin-like growth factor I (IGF-I) receptor-interacting protein, modulates IGF-I-dependent integrin signaling and promotes cell spreading and contact with extracellular matrix. Mol Cell Biol. 2002; 22(7):2345-2365. (Biology: Immunoprecipitation, Western blot). View Reference
  3. Kook S, Shim SR, Choi SJ, et al . Caspase-mediated cleavage of p130cas in etoposide-induced apoptotic Rat-1 cells. Mol Biol Cell. 2000; 11(3):929-939. (Biology: Western blot). View Reference
  4. Nagashima K, Endo A, Ogita H, et al. Adaptor protein Crk is required for ephrin-B1-induced membrane ruffling and focal complex assembly of human aortic endothelial cells. Mol Biol Cell. 2002; 13(12):4231-4242. (Biology: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  5. Sakai R, Iwamatsu A, Hirano N, et al. A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner. EMBO J. 1994; 13(16):3748-3756. (Biology). View Reference
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610271 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.