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      Purified Rat Anti-Mouse IgG2a
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
      Pooled mouse Ig
      ELISA Capture (Routinely Tested)
      0.5 mg/ml
      380793
      AB_394864
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      For the sandwich IgG2a ELISA, R11-89 mAb is optimal for capture with biotin-, AKP-, or HRP-conjugated anti-mouse IgG2a R19-15 mAb (Cat. No. 553388, 553389, or 553391, respectively) for detection. This pair of anti-mouse IgG2a mAbs can effectively quantitate mouse IgG2a of these mouse Igh-C haplotypes, in order of sensitivity from highest to lowest: e, a, j, and b.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      553446 Rev. 6
      Antibody Details
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      R11-89

      The R11-89 antibody recognizes mouse IgG2a, with strong reactivity to the Igh-1[a] allotype and weaker reactivity to Igh-1[b]. It does not react with other Ig isotypes. Molecular genetic analyses suggest that the Igh-I[b] allele, which encodes IgG2a[b], is derived from a locus found in several wild mouse subspecies, but not domestic mice, which encodes the IgG2c isotype.

      553446 Rev. 6
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      553446 Rev.6
      Citations & References
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      View product citations for antibody "553446" on CiteAb

      Development References (2)

      1. Martin RM, Silva A, Lew AM. The Igh-1 sequence of the non-obese diabetic (NOD) mouse assigns it to the IgG2c isotype. Immunogenetics. 1997; 46(2):167-168. (Biology). View Reference
      2. Morgado MG, Cam P, Gris-Liebe C, Cazenave PA, Jouvin-Marche E. Further evidence that BALB/c and C57BL/6 gamma 2a genes originate from two distinct isotypes. EMBO J. 1989; 8(11):3245-3251. (Biology). View Reference
      553446 Rev. 6

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.