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Product Details
BD Pharmingen™
Igh-4; Ighg1; Immunoglobulin heavy constant gamma 1
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
Mouse IgG1, κ Antibody
ELISA (Routinely Tested)
AB_396612
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with AKP under optimum conditions, and unconjugated antibody and free AKP were removed.
Recommended Assay Procedures
This antibody has been tested as a detecting reagent in ELISA (1:1000 dilution) to assure specificity and reactivity. For the sandwich AKP Rat Anti-Mouse IgG1 is optimal for detection with Purified Rat Anti-Mouse IgG1 (Cat. No. 553445) for capture.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
557272 Rev. 9
Antibody Details
X56
The X56 monoclonal antibody specifically binds to mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not crossreact with other Ig isotypes.
557272 Rev. 9
Format Details
AKP
Alkaline phosphatase (AKP) is an enzyme that is capable of dephosphorylating various substrates which generates a measurable colored product. Antibodies, avidin, streptavidin and other specific binding molecules can be conjugated with AKP and used in applications such as i) ELISA that employs a plate reader to measure absorbance of the colored product or ii) ELISPOT, Immunohistochemistry (IHC) or Western blot in which a colored product can be measured using light microscopy or image analysis.
AKP
557272 Rev.9
Citations & References
View product citations for antibody "557272" on CiteAb
Development References (2)
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Hao Z, Duncan GS, Su YW, et al.. The E3 ubiquitin ligase Mule acts through the ATM-p53 axis to maintain B lymphocyte homeostasis. J Exp Med. 2012; 209(1):173-186. (Methodology: ELISA). View Reference
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Kita-Furuyama M, Nagayama Y, Pichurin P, McLachlan SM, Rapoport B, Eguchi K. Dendritic cells infected with adenovirus expressing the thyrotrophin receptor induce Graves' hyperthyroidism in BALB/c mice. Clin Exp Immunol. 2003; 131(2):234-240. (Methodology: ELISA). View Reference
557272 Rev. 9
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.