Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Belgium (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      FITC Rat Anti-Mouse Vα2 TCR
      FITC Rat Anti-Mouse Vα2 TCR
      Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2  (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.
      FITC Rat Anti-Mouse Vα2 TCR
      Flow cytometric analysis of Vα2 TCR expression on mice peripheral lymphocytes. BALB/c lymph node cells were incubated simultaneously with PE Rat Anti-Mouse CD8b.2  (Cat. No. 553041), PE Rat Anti-Mouse CD4 (Cat. No. 553730/561829/557308) and FITC Rat Anti-Mouse Vα2 TCR (Cat. No. 553288/562085). The contour plot depicting CD4 versus Vα2 TCR expression was derived from gated events with the side and forward light-scatter characteristics of viable lymphocytes. Flow cytometry was performed on a FACScan™ system.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      T cell receptor alpha, variable region 2
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, λ
      Soluble αβ TCR from mouse cytotoxic T-cell clone KB5-C20
      Flow cytometry (Routinely Tested)
      0.5 mg/ml
      111660
      AB_394759
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      562085 Rev. 2
      Antibody Details
      Down Arrow Up Arrow
      B20.1

      The B20.1 monoclonal antibody specifically binds to most members of the Vα2 T-cell Receptor (TCR) subfamily in mice having the a, b, and c haplotypes of the Tcrb gene complex. B20.1 antibody may crossreact with Vδ8 TCR, which shares >90% sequence homology with Vα2 TCR. Levels of B20.1+ T cells appear to be influenced by Vα haplotypes. Moreover, the frequencies of Vα2+ CD8+ and CD4+ T cells are influenced by H-2 haplotypes.

      For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.

      562085 Rev. 2
      Format Details
      Down Arrow Up Arrow
      FITC
      Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      FITC
      Blue 488 nm
      494 nm
      518 nm
      562085 Rev.2
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "562085" on CiteAb

      Development References (3)

      1. Grégoire C, Rebaï N, Schweisguth F, et al. Engineered secreted T-cell receptor alpha beta heterodimers.. Proc Natl Acad Sci USA. 1991; 88(18):8077-81. (Clone-specific). View Reference
      2. Pircher H, Rebaï N, Groettrup M, et al. Preferential positive selection of V alpha 2+ CD8+ T cells in mouse strains expressing both H-2k and T cell receptor V alpha a haplotypes: determination with a V alpha 2-specific monoclonal antibody.. Eur J Immunol. 1992; 22(2):399-404. (Immunogen). View Reference
      3. Tomonari K, Fairchild S, Rosenwasser OA. Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection. Immunol Rev. 1993; 131:131-168. (Biology). View Reference
      562085 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.