BD FACSCelesta™

Working Together in Perfect Harmony

The BD FACSCelesta™ flow cytometer is designed to make multicolor flow cytometry more accessible and allow researchers to benefit from new innovations in instrument and reagent technology. Parallel advances in optical and reagent technology are pairing multilaser, multidetector instruments with bright new dyes, enabling increasingly deep and powerful insights in cell analysis.

For a limited time, purchase the BD FACSCelesta for a promotional price.

Optics Fluidics Applications Analysis

Optimized for Brilliance in a Compact Footprint

BD multi-laser flow cytometers are known for delivering high sensitivity and performance. In the BD FACSCelesta, the optical and electronics system—lasers, filters, detectors, optical paths, and signal processing technologies—have been engineered to get the most out of BD Horizon Brilliant™ dyes.

More Parameters Mean More Impact

BD FACSCelesta users can choose from four instrument configurations. All configurations have blue (488-nm) and violet (405-nm) lasers, which can be paired with a red (640-nm), ultraviolet (355-nm), or yellow-green (561-nm) laser, depending on your application needs. The ability to detect and analyze up to 14 parameters in a single sample maximizes your power of discovery and the impact of your research.

The laser combinations mix new and established technology to achieve optical stability and reliable performance within a compact footprint. A system of dichroic mirrors and bandpass filters, packed into a compact polygon, splits off light from each laser and directs it to the correct detectors. The detectors (photomultiplier tubes or PMTs) themselves fit compactly behind the filter assemblies.

Reliable Fluidics, High-Throughput Capability

The fluidics system of the BD FACSCelesta-the sample lines, fluid containers, and pumps that carry sample, sheath fluid, and waste through the unit-is designed for reliability and convenience. A newly designed digital control panel makes it easy to monitor the operational status of the cytometer and make adjustments. The simple display indicates the operational mode of the system.

BD FACSFlow Supply System Option

The optional BD FACSFlow™ Supply System (FFSS) fluidics cart increases capacity and ease of use while maintaining a stable fluidics pressure. It includes an automated sheath and waste fluid control system that reduces daily maintenance by incorporating two 20-L containers (Cubitainers®). Fluidic sensors maintain constant pressure, and a fluidics monitoring system warns when sheath fluid is low or empty, or when the waste container is full.

High-Throughput Option

For multi-sample applications requiring a high-throughput, workflow, the BD™ High Throughput Sampler (HTS) option for the BD FACSCelesta provides rapid, fully automated sample acquisition from microtiter plates. The HTS option supports a wide range of research applications and is compatible with 96-well U, V, and flat-bottom plates as well as 384-well microtiter plates. In high-throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes with less than 1% carryover. Standard throughput mode can be selected to acquire larger sample volumes or where longer acquisition times are required.

Immunology Applications

See What You've Been Missing

The BD FACSCelesta can rapidly and accurately analyze many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood.

Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation. The multiparametric, single-cell focus of multicolor flow cytometry is perfectly suited to further immunological discovery of protein and gene expression and cell signaling. BD's solutions for cell identification, transcription factor expression, and cytokine secretion and measurement reflect a commitment to high quality and consistency, which are needed for advanced research.

Illuminate Rare Cells and Low-Density Antigens

Rare cells, or cells that have few surface receptors of a marker of interest, can be difficult to detect using conventional reagents. Bright reagents are essential in resolving these dim cells from others in a sample.

BD Horizon Brilliant™ polymer conjugates can endow previously dim cells with much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC. Optimization for these bright dyes enables the BD FACSCelesta to identify cell populations with a broader range of receptor density than was previously possible.


Cell Biology Applications

Cell Analysis in a New Light

Flow cytometry can provide rich data to cell biologists working in a wide range of fields, from molecular interaction to systems biology, from pharmacokinetics to cancer biology, from cell signaling to marine biology to biophysics. Use of flow cytometry has blossomed with intensified interest in proteomics, increased use of biomarkers in drug development research, and the spread of high-throughput, cell-based drug screening research.

Detecting and analyzing light scatter allows researchers to measure physical characteristics of cells in suspension, such as cell shape, size, and internal complexity. Adding fluorescent markers allows researchers to interrogate expressed or secreted proteins that reveal cell phenoptype, function, and status. Using a broad landscape of cell function assays, flow cytometry can shed light on a variety of sample types, such as whole blood, cell lines, and yeast. With three lasers and up to 14 optical channels, the BD FACSCelesta can multiplex many of these assays at once on the same sample.

Study Complex Populations at the Single-Cell Level

A major strength of flow cytometry is its ability to study complex populations. Western blots, immunoprecipitation, and PCR-based techniques rely on lysing the entire sample. Their results provide useful data for the population as a whole, but it can be difficult to compare subsets of cells that might behave differently. Other techniques that examine individual cells, such as microscopy, are hard to scale up for quantitative analysis. Flow cytometry can characterize large numbers of individual cells, allowing it to identify, quantify, and characterize different subsets of cells in a heterogeneous population.

Streamlined Workflows from Setup Through Analysis

The BD FACSCelesta operates with BD FACSDiva™ software, a collection of convenient and easy tools for flow cytometer and application setup, data acquisition, and data analysis. BD FACSDiva helps streamline flow cytometry workflows, expanding flexibility, and improving efficiency in today's busy laboratory.

The software package simplifies the creation and management of samples and experiments, and provides a wide array of analysis features for laboratories through use of a single software platform. Efficient data management tools simplify data storage and improve data organization, while allowing the flexibility to export data for use with downstream analysis and presentation application.

Intuitive Workflows Help You Focus on Science

With a large worldwide installed base spanning multiple platforms, BD FACSDiva is already familiar to many users, reducing the need for training. Maintaining consistency and accuracy of data acquisition requires only a single daily setup and QC run using BD FACSDiva software and BD  FACSDiva™ CS&T (Cytometer Setup & Tracking) research beads. The CS&T software module enables BD FACSDiva to automatically calculate and adjust key cytometer setup values for PMT voltages, laser delay, and area scaling factors, thereby sustaining optimal performance over time and across instruments. This automation reduces startup time to approximately five minutes and eliminates multiple error-prone and expensive manual data acquisitions and calculations.

Reagents Instruments
Immunology Cell Biology

Overview

The BD FACSCelesta™ flow cytometer can rapidly and accurately analyze many kinds of cells, including lymphoid tissue (thymus, spleen and lymph nodes), digested solid tissues and blood.

Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation. The multiparametric, single-cell focus of multicolor flow cytometry is perfectly suited to further immunological discovery of protein and gene expression and cell signaling. BD's solutions for cell identification, transcription factor expression, and cytokine secretion and measurement reflect a commitment to high quality and consistency, which are needed for advanced research.

Illuminate Rare Cells and Low-Density Antigens

Rare cells, or cells that have few surface receptors of a marker of interest, can be difficult to detect using conventional reagents. Bright reagents are essential in resolving these dim cells from others in a sample.

BD Horizon Brilliant™ polymer conjugates can endow previously dim cells with much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC. Optimization for these bright dyes enables the BD FACSCelesta to identify cell populations with a broader range of receptor density than was previously possible.

2-Laser BV Configuration

Ten-color T-cell analysis on the BD FACSCelesta BV configuration

3-Laser BVR Configuration

Six-color Treg analysis on the BD FACSCelesta BVR configuration

Ten-color T-cell analysis on the BD FACSCelesta BVR configuration

Three-color minimal spectral overlap human Treg panel on the BD FACSCelesta BVR configuration

Resolution of T-cell markers on the BD FACSCelesta BVR configuration

Deep immunophenotyping of mouse dendritic cell subsets

3-Laser BVYG Configuration

Eight-color NK and Treg analysis on the BD FACSCelesta BVYG configuration

3-Laser BVUV Configuration

Six-color Treg analysis on the BD FACSCelesta BVUV configuration

Twelve-color T-cell analysis on the BD FACSCelesta BVUV configuration

Four-color minimal spectral overlap human Treg panel on the BD FACSCelesta BVUV configuration

Resolution of B-cell markers on the BD FACSCelesta BVUV configuration

Intracellullar cytokine response in TLR ligand-activated human dendritic cell subsets

Cell Analysis in a New Light

Flow cytometry can provide rich data to cell biologists working in a wide range of fields, from molecular interactions to systems biology, from pharmacokinetics to cancer biology, from cell signaling to marine biology to biophysics. Use of flow cytometry has blossomed with intensified interest in proteomics, increased use of biomarkers in drug development research and the spread of high-throughput, cell-based drug screening research.

Detecting and analyzing light scatter allows researchers to measure physical characteristics of cells in suspension, such as cell shape, size and internal complexity. Adding fluorescent markers allows researchers to interrogate expressed or secreted proteins that reveal cell phenotype, function and status. Using a broad landscape of cell function assays, flow cytometry can shed light on a variety of sample types, such as whole blood, cell lines and yeast. With three lasers and up to 14 optical channels, the BD FACSCelesta™ flow cytometer can multiplex many of these assays at once on the same sample.

Study Complex Populations at the Single-cell Level

A major strength of flow cytometry is its ability to study complex populations. Western blots, immunoprecipitation and PCR-based techniques rely on lysing the entire sample. Their results provide useful data for the population as a whole, but it can be difficult to compare subsets of cells that might behave differently. Other techniques that examine individual cells, such as microscopy, are hard to scale up for quantitative analysis. Flow cytometry can characterize large numbers of individual cells, allowing it to identify, quantify and characterize different subsets of cells in a heterogeneous population.

3-Laser BVR Configuration

Two-color flow cytometric analysis of apoptosis and viability in Jurkat cells

Minimal spectral overlap panel for cell cycle analysis on the BD FACSCelesta

Multiplexed cell cycle and immunophenotyping analysis on the BD FACSCelesta

3-Laser BVYG Configuration

Expression of fluorescent proteins in transfected human embryonic kidney cells


For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Class 1 Laser Product.
APC-Cy7: US patent 5,714,386
CF™ is a trademark of Biotium, Inc.
Living Colors® (including mCherry dye) is a registered trademark of Clontech.
Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
Cubitainer is a registered trademark of Hedwin Corporation.
SPHERO™ is a trademark of Spherotech, Inc.