-
Your selected country is
Italy
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
Western blot analysis of TEF-1 on A431 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of TEF-1.
3T3-L1
BD Transduction Laboratories™ Purified Mouse Anti-TEF-1
BD Transduction Laboratories™ Purified Mouse Anti-TEF-1
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
TEF-1 (transcription enhancer factor-1) is a member of a family of transcription factors that contain an evolutionarily conserved DNA binding domain (DBD), TEA/ATTS. TEF-1 was identified as a HeLa cell transcriptional activator that bound, via its TEA domain, to the GT-IIC and Sph enhancer elements. Comparison of various enhancers indicates that TEF-1 binds to highly degenerate DNA sequences and cooperatively interacts with tandem repeats of its binding sites, which increases its enhancer activity. However, the DNA binding activities of TEF-1 are modulated by sequences outside the DBD. The N-terminal basic portion of TEF-1 contains the DBD. The C-terminal portion contains the transactivation domain and a zinc finger-like motif that is thought to be involved in protein dimerization or interaction with additional transcription factors. TEF-1 induced transcription requires a limiting transcriptional intermediary factor (TIF) and a TATA-binding protein associated factor (hTAFIIs). Although TEF-1 is expressed ubiquitously during embryogenesis, its expression is limited to adult kidney, heart, brain, skeletal muscle, and lungs. Disruption of murine TEF-1 gene results in fetal death.
Development References (5)
-
Deshpande N, Chopra A, Rangarajan A, Shashidhara LS, Rodrigues V, Krishna S. The human transcription enhancer factor-1, TEF-1, can substitute for Drosophila scalloped during wingblade development. J Biol Chem. 1997; 272(16):10664-10668. (Biology). View Reference
-
Gupta MP, Amin CS, Gupta M, Hay N, Zak R. Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression. Mol Cell Biol. 1997; 17(7):3924-3936. (Biology). View Reference
-
Jacquemin P, Hwang JJ, Martial JA, Dolle P, Davidson I. A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain. J Biol Chem. 1996; 271(36):21775-21785. (Biology). View Reference
-
Ueyama T, Zhu C, Valenzuela YM, Suzow JG, Stewart AF. Identification of the functional domain in the transcription factor RTEF-1 that mediates alpha 1-adrenergic signaling in hypertrophied cardiac myocytes. J Biol Chem. 2000; 275(23):17476-17480. (Clone-specific: Gel shift). View Reference
-
Xiao JH, Davidson I, Matthes H, Garnier JM, Chambon P. Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1. Cell. 1991; 65(4):551-568. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.