BD Pharmingen™ Purified Mouse Anti-Pig IFN-γ

ELISA Capture: The purified P2G10 antibody (Cat. No. 559961) is useful as a capture antibody in a sandwich ELISA for measuring pig IFN-γ protein levels. Purified P2G10 antibody can be paired with the biotinylated P2C11 antibody (Cat. No.559958) as the detecting antibody. Purified P2G10 antibody should be titrated 1-4 µg/ml to determine optimal concentration for ELISA capture. To obtain linear standard curves, doubling dilutions of pig IFN-γ ranging from ~500 to 4 pg/ml are recommended for inclusion in each ELISA plate. For specific methodology please visit the protocols section or the chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

Immunofluorescence and Flow cytometry: For immunofluorescent staining and flow cytometric analysis, the P2G10 antibody has been found useful to identify and enumerate IFN-γ producing cells within mixed cell populations. PE- conjugated P2G10 antibody (Cat. No. 559812) is especially suitable for these studies. For specific methodology, please visit the protocols section or chapter on intracellular staining in the Immune Function Handbook, both of which are posted on our web site, www.bdbiosciences.com.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.