Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel: Lysates of the AR42J cell line (ATCC CRL-1492) were electrophoresed (SDS-PAGE) in a 4-20% Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 (lane 1), 1 (lane 2), 0.5 (lane 3), 0.25 (lane 4) mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Right panel: AR42J cells were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line) or Purified Mouse anti-PTF1A monoclonal antibody (solid line). The second-step reagent was PE Goat Anti-Mouse Ig (550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; left panel) or Purified Mouse anti-PTF1A antibody (right panel). A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin- HRP (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40×.
Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel: Lysates of the AR42J cell line (ATCC CRL-1492) were electrophoresed (SDS-PAGE) in a 4-20% Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 (lane 1), 1 (lane 2), 0.5 (lane 3), 0.25 (lane 4) mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Right panel: AR42J cells were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line) or Purified Mouse anti-PTF1A monoclonal antibody (solid line). The second-step reagent was PE Goat Anti-Mouse Ig (550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878; left panel) or Purified Mouse anti-PTF1A antibody (right panel). A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin- HRP (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40×.
Product Details
BD Pharmingen™
PTF1α; Ptf1a; Ptf1α; bHLHa29; PTF1-p48
Mouse (QC Testing), Human,Rat (Tested in Development)
Mouse IgG1, κ
Human PTF1A Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence, Immunohistochemistry-formalin (antigen retrieval required), Western blot (Tested During Development)
48 kDa
0.5 mg/ml
AB_2738928
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Retrievagen A (pH 6.0) RUO
Cat No.
550524
Purified Mouse IgG1 κ Isotype Control RUO
Size
0.1 mg
Cat No.
554121
564745 Rev. 2
Antibody Details
S25-763
The S25-763 monoclonal antibody specifically binds PTF1A (Pancreas specific transcription factor 1a). PTF1A is a transcription factor that contains a basic helix-loop-helix motif domain and, in combination with p75 and p64, forms the pancreas transcription factor complex. PTF1A plays a roles in pancreatic and cerebellar development. In early pancreatic development, pancreatic bud cells can either continue to pancreatic organogenesis or regress to a duodenal fate, and PTF1A plays a role in this differentiation. In late pancreatic development, the expression of PTF1A is restricted to acinar cells where it is involved in the maintenance of exocrine-specific gene expression. PTF1A expression is often evaluated to monitor pancreatic lineage differentiation from pluripotent stem cells. In addition, PTF1A is expressed during retinal development, and mutations in PTF1A cause cerebellar deficiencies.
This mouse anti-human PTF1A monoclonal antibody, clone S25-763, has been demonstrated to cross-react with rat and mouse pancreatic acinar cells.
564745 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
564745 Rev.2
Citations & References
View product citations for antibody "564745" on CiteAb
Development References (7)
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Kelly OG, Chan MY, Martinson LA, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011; 29(8):750-756. (Biology). View Reference
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Krapp A, Knöfler M, Ledermann B, et al. The bHLH protein PTF1-p48 is essential for the formation of the exocrine and the correct spatial organization of the endocrine pancreas. Genes Dev. 1998; 12(23):3752-3763. (Biology). View Reference
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Masui T, Swift GH, Hale MA, Meredith DM, Johnson JE, Macdonald RJ. Transcriptional autoregulation controls pancreatic Ptf1a expression during development and adulthood. Mol Cell Biol. 2008; 28(17):5458-5468. (Biology). View Reference
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Nakhai H, Sel S, Favor J, et al. Ptf1a is essential for the differentiation of GABAergic and glycinergic amacrine cells and horizontal cells in the mouse retina. Development. 2007; 134:1151-1160. (Biology). View Reference
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Pan FC, Bankaitis ED, Boyer D, et al. Spatiotemporal patterns of multipotentiality in Ptf1a-expressing cells during pancreas organogenesis and injury-induced facultative restoration. Development. 2013; 140(4):751-764. (Biology). View Reference
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Roux E, Strubin M, Hagenbüchle O, Wellauer PK. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 1989; 3(10):1613-1624. (Biology). View Reference
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Sellick GS, Barker KT, Stolte-Dijkstra I, et al. Mutations in PTF1A cause pancreatic and cerebellar agenesis. Nat Genet. 2004; 36(12):1301-1305. (Biology). View Reference
564745 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.