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R718 Rat Anti-Mouse CD107a (LAMP-1)
R718 Rat Anti-Mouse CD107a (LAMP-1)

Flow cytometric analysis of CD107a expression on mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histogram) or BD Horizon™ R718 Rat Anti-Mouse CD107a antibody (Cat. No. 566986; solid line histogram) at 0.5 µg/test. A histogram showing CD107a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Flow cytometric analysis of CD107a expression on mouse splenocytes. Mouse splenic leucocytes were fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and subsequently stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; dashed line histogram) or BD Horizon™ R718 Rat Anti-Mouse CD107a antibody (Cat. No. 566986; solid line histogram) at 0.5 µg/test. A histogram showing CD107a expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Flow Cytometer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.

Product Details
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BD Horizon™
LAMP-1; LGP-120; LGP-A; P2B
Mouse (QC Testing)
Rat SD, also known as Sprague-Dawley (outbred) IgG2a, κ
Plasma membrane fraction of mouse embryo NIH 3T3 cell line
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2869990
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® is a registered trademark of Life Technologies Corporation.
  5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  7. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
566986 Rev. 3
Antibody Details
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1D4B

The 1D4B antibody recognizes CD107a which is also known as, Lysosome-Associated Membrane Protein 1 (LAMP-1). CD107a is one of the two major glycoproteins in lysosome membranes that provide useful markers to distinguish lysosomes from other organelles. CD107a may play a role in the lysosomal degradation of certain molecules. Mouse CD107a is a type I transmembrane glycoprotein. It consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa.  It is principally expressed in epithelial cells and macrophages in a variety of organs. Following activation, CD107a is relocated to the surface of some lymphocytes, macrophages, epithelial cells, endothelial cells, platelets, and tumor cells. Cell-surface CD107a may participate in intercellular adhesion and adhesion to the extracellular matrix. Cell surface CD107a expression can serve as a useful marker for cytotoxic NK and CD8+ T cells, as well as, some malignant tumor cells.

The antibody was conjugated to BD Horizon Red 718, which has been developed exclusively for BD Biosciences as a better alternative to Alexa Fluor® 700. BD Horizon Red 718 can be excited by the red laser (628 – 640 nm) and, with an Em Max around 718 nm, it can be detected using a 730/45 nm filter. Due to similar excitation and emission properties, we do not recommend using R718 in combination with APC-R700 or Alexa Fluor® 700.

566986 Rev. 3
Format Details
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R718
The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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R718
Red 627-640 nm
695 nm
718 nm
566986 Rev.3
Citations & References
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Development References (7)

  1. Arterburn LM, Earles BJ, August JT. The disulfide structure of mouse lysosome-associated membrane protein 1. J Biol Chem. 1990; 265(13):7419-7423. (Clone-specific: Immunoaffinity chromatography). View Reference
  2. Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Clone-specific: Immunohistochemistry, Immunoprecipitation). View Reference
  3. Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Radioimmunoassay). View Reference
  4. Chen JW, Pan W, D'Souza MP, August JT. Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Arch Biochem Biophys. 1985; 239(2):574-586. (Immunogen: Fluorescence microscopy, Immunoaffinity chromatography, Immunofluorescence). View Reference
  5. Dressel R, Elsner L, Novota P, Kanwar N, Fischer von Mollard G. The exocytosis of lytic granules is impaired in Vti1b- or Vamp8-deficient CTL leading to a reduced cytotoxic activity following antigen-specific activation. J Immunol. 2010; 185(2):1005-1014. (Clone-specific: Flow cytometry, Functional assay). View Reference
  6. He JS, Ostergaard HL. CTLs contain and use intracellular stores of FasL distinct from cytolytic granules. J Immunol. 2007; 179(4):2339-2348. (Clone-specific: Flow cytometry). View Reference
  7. Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 1996; 132(4):565-576. (Clone-specific: Electron microscopy, Immunocytochemistry (cytospins)). View Reference
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566986 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.