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Purified Mouse anti-SLP-76 (pY113)
Purified Mouse anti-SLP-76 (pY113)

Western blot analysis of SLP-76 (pY113) in human T leukemia.  Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Jurkat cells were probed with purified mouse anti-SLP-76 (pY113) monoclonal antibody at concentrations of 0.0039 (lanes 1 and 4), 0.0019 (lanes 2 and 5),  and 0.0010 µg/ml (lanes 3 and 6).  SLP-76 (pY113) is identified as a band of 76 kDa in the treated cells.

Western blot analysis of SLP-76 (pY113) in human T leukemia.  Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Jurkat cells were probed with purified mouse anti-SLP-76 (pY113) monoclonal antibody at concentrations of 0.0039 (lanes 1 and 4), 0.0019 (lanes 2 and 5),  and 0.0010 µg/ml (lanes 3 and 6).  SLP-76 (pY113) is identified as a band of 76 kDa in the treated cells.

Product Details
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BD Pharmingen™
Human (QC Testing)
Mouse BALB/c IgG1, κ
Phosphorylated Human SLP-76 Peptide
Western blot (Routinely Tested)
76 kDa
0.5 mg/ml
AB_647329
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
558388 Rev. 4
Antibody Details
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J80-373

SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway.  It may be involved in the signaling pathways of other peripheral blood leukocytes; thymic/splenic cells; and in human T, B, and monocytic cell lines.  SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region.  SLP-76 has been shown to associate with Gads, Grb2, PLCγ1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes.  An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors.  The phosphorylated tyrosine 113 (Y113) brings into the signaling complex the Rho-family guanine-nucleotide exchange factor Vav1, which is involved in the formation of a multimolecular assembly that participates in TCR-stimulated actin cytoskeletal rearrangement.  

The J80-373 monoclonal antibody recognizes the phosphorylated Y113 of activated SLP-76.

558388 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558388 Rev.4
Citations & References
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Development References (3)

  1. Fang N, Motto DG, Ross SE, Koretzky GA. Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity. J Immunol. 1996; 157:3769-3773. (Biology). View Reference
  2. Janssen E, Zhang W. Adaptor proteins in lymphocyte activation. Curr Opin Immunol. 2003; 15:269-276. (Biology). View Reference
  3. Wu JN, Koretzky GA. The SLP-76 family of adapter proteins. Semin Immunol. 2004; 16:379-393. (Biology). View Reference
558388 Rev. 4

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.