Skip to main content Skip to navigation
Hamburger Menu
Search icon
Mini Cart
Empty CartYou are not signed in.
Country Selector Country Selector
Finland (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Mini Cart
Empty CartYou are not signed in.
Products

Link Arrow
Solutions

Link Arrow
Discover & Learn

Link Arrow
Resources & Tools

Link Arrow
Support

Link Arrow
Search icon Search icon
Close Menu
    Mini Cart
    Empty CartYou are not signed in.
    Expand menu Expand menu
      Loading...
      Alert Icon
      Purified Mouse Anti-CREB (pS133) / ATF-1 (pS63)
      558359_image_1.png
      558359_image_1.png
      clear-yellow-white.jpg
      PE-Texas_Red.jpg

      Western blot analysis of CREB (pS133) / ATF-1 (pS63).  Lysates from control (left panel) and PMA-activated (right panel) Jurkat cells (Human T-cell leukemia; ATCC TIB-152) were probed with the purified mouse anti-CREB (pS133) / ATF-1 (pS63) antibody at concentrations of 0.25 (lanes 1 and 4), 0.125 (lanes 2 and 5), and 0.06 µg/ml (lanes 3 and 6). CREB (pS133) is identified as a band of 43 kDa in the PMA-treated cells. Another band migrating below it has been identified as ATF-1 (pS63).  Treatment with lambda phosphatase removes all bands in blots of control and PMA-activated lysates (data not shown), demonstrating the antibody's specificity for the indicated phosphorylated proteins.

      Show More
      558359
      Sign In
      EA (1 Each)
      0.1 mg
      Add to Cart IconAdd to Cart
      Save To Shopping ListRequest Bulk Reagent Quote
      Product Details
      Down Arrow


      BD Pharmingen™
      Human (QC Testing), Mouse, Rat (Predicted)
      Mouse BALB/c IgG1, κ
      Phosphorylated Human CREB Peptide
      Western blot (Routinely Tested)
      43 kDa
      0.5 mg/ml
      AB_647270
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow

      Data Sheets

      DownloadTechnical Data Sheet (TDS)
      558359 Rev. 3
      Antibody Details
      Down Arrow
      J151-21

      Transcription of various genes is regulated by the cyclic AMP (cAMP) signal transduction pathway through a family of cAMP Responsive Element (CRE)-Binding transcription factors that include CREB, CREM (CRE Modulator), and ATF-1 (Activating Transcription Factor 1).  The genes for these transcription factors encode multiple isoforms that are created by alternative splicing, alternative initiation codons, and alternative intronic promoters.  Homo- and heterodimers of these members of the basic domain-and-leucine zipper superfamily of proteins bind the palindromic TGACGTCA sequence of CRE.  CREB is expressed in all somatic cells, and many different stimuli activate CREB by phosphorylation of its serine 133 (S133).  The transcriptional activity and specificity of CREB are regulated by the signaling pathways initiated by the various stimuli.  Transcriptional regulation by CREB family members has been implicated in many physiological responses to extracellular and environmental stimuli, such as memory, tissue growth and development, and homeostasis.

      The J151-21 monoclonal antibody recognizes the phosphorylated S133 of human CREB in addition to cross-reacting with S63 of human ATF-1.

      558359 Rev. 3
      Format Details
      Down Arrow
      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      558359 Rev.3
      Citations & References
      Down Arrow

      Development References (1)

      1. Shaywitz AJ, Greenberg ME. CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem. 1999; 68:821-861. (Biology).
      558359 Rev. 3

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.