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Purified Mouse Anti-Acid Ceramidase
Product Details
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BD Transduction Laboratories™
Human (QC Testing), Dog (Tested in Development)
Mouse IgG1
Human Acid Ceramidase aa. 88-182
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
13 kDa
250 µg/ml
AB_399617
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
612302 Rev. 1
Antibody Details
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23/Acid Ceramidase

Ceramide is a sphingolipid that exhibits a wide variety of functions, including monocyte differentiation, apoptosis, neurite outgrowth, and Ca2+ transport. It also serves as the precursor of many sphingolipids and anchors these into the outer leaflet of the plasma membrane via hydrophobic interactions. Acid ceramidase is a lysosomal enzyme that was purified from human urine. It is synthesized as a 55kDa precursor protein, which is then processesed into the mature α-subunit (13kDa) and β-subunit (40kDa). Acid ceramidase catalyzes the hydrolysis of ceramide into free fatty acid and sphingosine. Sphingosine, and its phosphorylated form, sphingosine-1-phosphate (SPP), have been shown to inhibit PKC activity and act as a second messenger in cell proliferation and differentiation. Acid ceramidase is also the cause of a lysosomal storage disorder known as Farber's disease. This disease is characterized by an accumulation of ceramide in tissues, leading to swelling and pain of the joints and extremities, pulmonary insufficiency, and death at an early age. Thus, acid ceramidase is necessary in the hydrolysis of ceramide and is the cause of Farber's disease.

612302 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612302 Rev.1
Citations & References
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Development References (3)

  1. Bernardo K, Hurwitz R, Zenk T, et al. Purification, characterization, and biosynthesis of human acid ceramidase. J Biol Chem. 1995; 270(19):11098-11102. (Biology). View Reference
  2. Ferlinz K, Kopal G, Bernardo K, et al. Human acid ceramidase: processing, glycosylation, and lysosomal targeting. J Biol Chem. 2001; 276(38):35352-35360. (Biology). View Reference
  3. Strelow A, Bernardo K, Adam-Klages S, et al. Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death. J Exp Med. 2000; 192(5):601-611. (Biology). View Reference
612302 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.