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Western blot analysis of CD3ζ. Lysate from Jurkat cells was probed with anti-CD3ζ (clone 8D3, Comp. No. 51-6527GR) at concentrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 µg/ml (lane 3). CD3ζ is identified as a band of 16 kDa.
BD Pharmingen™ Purified Mouse Anti-Human CD247 with Control
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multi-component complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes Ti is present as a disulfide-linked α-β heterodimer. A minor fraction express Ti as a γ-δ heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains, γ (gamma), δ (delta), ε (epsilon), ζ (zeta), and p21. The relative masses of the mouse CD3 chains are 21, 28, 26, 32 (dimer), and 21 kDa respectively.
The 8D3 antibody recognizes the ζ chain of the human and mouse T cell antigen receptor-associated CD3 complex. It reacts with 16 kDa unphosphorylated monomers and 32 kDa unphosphorylated dimers. It does not appear to recognize 21 kDa phosphorylated monomers or 42 kDa phosphorylated dimers. A synthetic peptide corresponding to a region of human CD3ζ was used as immunogen.
Preparation And Storage
Recommended Assay Procedures
Jurkat control lysate [50 µg (1 µg/µl) is provided as a Western blot positive control (Comp. 51-16526N; store lysate at -20°C). Additional control lysate (Cat. No. 611451) is sold separately as a ready-to-use western blot control.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Description | Quantity/Size | Part Number | EntrezGene ID |
---|---|---|---|
Purified Mouse Anti-Human CD247 | 50 µg (1 ea) | 51-6527GR | N/A |
Jurkat Cell Lysate | 50 µg (1 ea) | 51-16526N | N/A |
Development References (1)
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Hussey RE, Clayton LK, Diener A, et al. Overexpression of CD3 eta during thymic development does not alter the negative selection process. J Immunol. 1993; 150(4):1183-1194. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.