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BD Transduction Laboratories™ Purified Mouse Anti-Pax-5
Clone 24/Pax-5 (RUO)
Western blot analysis of Pax-5 on mouse spleen lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Pax-5.
Pax-5 (clone 24) staining on human tonsil. Formalin fixed paraffin section with citrate buffer pretreatment. 10x.
Pax-5 staining on paraformaldehyde-fixed SW-13 cells.
Western blot analysis of Pax-5 on mouse spleen lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of Pax-5.
Pax-5 (clone 24) staining on human tonsil. Formalin fixed paraffin section with citrate buffer pretreatment. 10x.
Pax-5 staining on paraformaldehyde-fixed SW-13 cells.
Product Details
BD Transduction Laboratories™
Mouse (QC Testing), Human (Tested in Development)
Mouse IgG1
Human Pax-5 aa. 151-306
Western blot (Routinely Tested), Immunofluorescence, Immunohistochemistry (Tested During Development), Immunoprecipitation (Not Recommended)
50 kDa
250 µg/ml
AB_398182
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610862 Rev. 2
Antibody Details
24/Pax-5
There are at least nine members of the paired box (Pax) gene family whose protein products are transcription factors involved in development. The conserved paired box DNA-binding domain is found in the N-terminal region of Pax proteins. An octomer and homeodomain sequence are conserved in the center of the proteins. The Ser/Thr/Pro-rich region in the C-terminal portion contains a conserved 100 amino acid transactivating domain. One of the best studied Pax family members, Pax 5, is a B cell specific activator protein (BSAP). In the early stages of B cell development, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. Pax-5 has been found to be important in both B cell and nervous system development.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
610862 Rev. 2
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610862 Rev.2
Citations & References
View product citations for antibody "610862" on CiteAb
Development References (5)
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Adams B, Dorfler P, Aguzzi A, et al. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis. Genes Dev. 1992; 6(9):1589-1607. (Biology). View Reference
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Foss HD, Reusch R, Demel G, et al. Frequent expression of the B-cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin's disease provides further evidence for its B-cell origin. Blood. 1999; 94(9):3108-3113. (Clone-specific: Immunohistochemistry). View Reference
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Hertel CB, Zhou XG, Hamilton-Dutoit SJ, Junker S. Loss of B cell identity correlates with loss of B cell-specific transcription factors in Hodgkin/Reed-Sternberg cells of classical Hodgkin lymphoma. Oncogene. 2002; 21(32):4908-4920. (Clone-specific: Immunohistochemistry). View Reference
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Klein U, Tu Y, Stolovitzky GA, et al. Transcriptional analysis of the B cell germinal center reaction. Proc Natl Acad Sci U S A. 2003; 100(5):2639-2644. (Clone-specific: Immunohistochemistry). View Reference
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Zwollo P, Arrieta H, Ede K, Molinder K, Desiderio S, Pollock R. The Pax-5 gene is alternatively spliced during B-cell development. J Biol Chem. 1997; 272(15):10160-10168. (Biology). View Reference
610862 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.