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Purified Mouse Anti-N-Cadherin
Purified Mouse Anti-N-Cadherin

Western blot analysis of N-Cadherin on HeLa lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000, lane 4: 1:2000 dilution of anti-N-Cadherin.

Purified Mouse Anti-N-Cadherin

Immunofluorescent staining of 293 cells with anti-N-Cadherin.

Western blot analysis of N-Cadherin on HeLa lysate.  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000, lane 4: 1:2000 dilution of anti-N-Cadherin.

Immunofluorescent staining of 293 cells with anti-N-Cadherin.

Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Chicken (Tested in Development)
Mouse IgG1
Mouse N-Cadherin aa. 802-819
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunoprecipitation (Reported)
130 kDa
250 µg/ml
AB_398236
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
610920 Rev. 1
Antibody Details
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32/N-Cadherin

Cadherins are a family of Ca2+-dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (α, β, γ). Members of the cadherin family include P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of β-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates β-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.

610920 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610920 Rev.1
Citations & References
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Development References (5)

  1. Bhowmick NA, Ghiassi M, Bakin A. Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. Mol Biol Cell. 2001; 12(1):27-36. (Clone-specific: Immunofluorescence, Western blot). View Reference
  2. Cowin P. Unraveling the cytoplasmic interactions of the cadherin superfamily. Proc Natl Acad Sci U S A. 1994; 91(23):10759-10761. (Biology). View Reference
  3. Izawa I, Nishizawa M, Ohtakara K, Inagaki M. Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses. J Biol Chem. 2002; 277(7):5345-5350. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
  4. Miyatani S, Shimamura K, Hatta M. Neural cadherin: role in selective cell-cell adhesion. Science. 1989; 245(4918):631-635. (Biology). View Reference
  5. Nurnberger J, Bacallao RL, Phillips CL. Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells. Mol Biol Cell. 2002; 13(9):3096-3106. (Clone-specific: Immunofluorescence, Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610920 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Non-IVD products are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

 

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