Skip to main content Skip to navigation
Purified Mouse Anti-Mouse Thrombospondin-2
Purified Mouse Anti-Mouse Thrombospondin-2
Western blot analysis of Thrombospondin-2 on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-mouse thrombospondin-2 antibody.
Western blot analysis of Thrombospondin-2 on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-mouse thrombospondin-2 antibody.
Product Details
Down Arrow Up Arrow


BD Transduction Laboratories™
TSP-2
Mouse (QC Testing)
Mouse IgG1
Human Thrombospondin-2 aa. 173-295
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
200 kDa
250 µg/ml
AB_398690
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611150 Rev. 1
Antibody Details
Down Arrow Up Arrow
4/Thrombospondin-2

Cell adhesion interactions involve large extracellular glycoproteins such as fibronectin, laminins, and thrombospondins.  The thrombospondins (TSPs), a small family of secreted glycoproteins, can be separated into two subfamilies based on structural similarity.  TSP-1 and TSP-2 are homotrimers of monomeric 145 kDa chains, while TSP-3,4 and 5 are homopentamers of lower MW chains.  TSP-1 and TSP-2 interact with several of the same cell surface receptors and have been termed matricellular proteins.  However, the functional roles of TSP-1 and TSP-2 are poorly understood.  Study of TSP-2 deficient mice has shed some light on the functional role of this protein.  Although these mice appear normal, they in fact exhibit a wide variety of irregularities.  These include abnormal collagen fiber development which results in fragile skin and highly flexible tendons and ligaments.  They also exhibit increased cortical thickness in long bones and abnormal bleeding time.  These results, together with the developmental expression pattern of TSP-2, suggest that it functions as a modulator of the cell surface properties of mesenchymal cells which, in turn, affects cell functions such as adhesion and migration.

This antibody is routinely tested by western blot analysis. Otehr applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

611150 Rev. 1
Format Details
Down Arrow Up Arrow
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611150 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (3)

  1. Kyriakides TR, Zhu YH, Smith LT, et al. Mice that lack thrombospondin 2 display connective tissue abnormalities that are associated with disordered collagen fibrillogenesis, an increased vascular density, and a bleeding diathesis. J Cell Biol. 1998; 140(2):419-430. (Biology). View Reference
  2. Kyriakides TR, Zhu YH, Yang Z, Bornstein P. The distribution of the matricellular protein thrombospondin 2 in tissues of embryonic and adult mice. J Histochem Cytochem. 1998; 46(9):1007-1015. (Biology). View Reference
  3. LaBell TL, Milewicz DJ, Disteche CM, Byers PH. Thrombospondin II: partial cDNA sequence, chromosome location, and expression of a second member of the thrombospondin gene family in humans. Genomics. 1992; 12(3):421-429. (Biology). View Reference
611150 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.