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Purified Mouse Anti-IP3R-3
Purified Mouse Anti-IP3R-3
Western blot analysis of IP3R-3 on a HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- IP3R-3 antibody.
Purified Mouse Anti-IP3R-3
Immunofluorescence staining of MDCK cells (canine kidney; ATCC CCL-34).
Western blot analysis of IP3R-3 on a HeLa cell lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti- IP3R-3 antibody.
Immunofluorescence staining of MDCK cells (canine kidney; ATCC CCL-34).
Product Details
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BD Transduction Laboratories™
Inositol 1,4,5-triphosphate receptor type III
Human (QC Testing), Mouse, Rat, Dog, Cow (Tested in Development)
Mouse IgG2a
Human IP3R-3 aa. 22-230
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
300 kDa
250 µg/ml
AB_397705
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610313 Rev. 1
Antibody Details
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2/IP3R-3

Inositol 1,4,5-triphosphate (IP3) functions as a second messenger for many hormones, growth factors, and neurotransmitters. IP3 causes the release of Ca2+ from intracellular stores by binding specific receptors that are coupled to Ca2+ channels. A number of studies have identified a family of at least four IP3 receptors (IP3R). The type III receptor (IP3R-3) has been isolated and characterized in human and rat. IP3 receptors are commonly localized in the endoplasmic reticulum, but have also been identified in the nucleus and the plasma membrane. Co-expression of different IP3 receptors is detected in most tissues and cell lines. Although these receptors appear to have a similar specificity for inositol phosphates, the different receptors have been reported to have different affinities for IP3 as follows: type II > type I > type III.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

610313 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610313 Rev.1
Citations & References
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Development References (5)

  1. Blondel O, Takeda J, Janssen H, Seino S, Bell GI. Sequence and functional characterization of a third inositol trisphosphate receptor subtype, IP3R-3, expressed in pancreatic islets, kidney, gastrointestinal tract, and other tissues. J Biol Chem. 1993; 268(15):11356-11363. (Biology). View Reference
  2. Leite MF, Thrower EC, Echevarria W, et al. Nuclear and cytosolic calcium are regulated independently. Proc Natl Acad Sci U S A. 2003; 100(5):2975-2980. (Biology: Immunofluorescence, Western blot). View Reference
  3. Maranto AR. Primary structure, ligand binding, and localization of the human type 3 inositol 1,4,5-trisphosphate receptor expressed in intestinal epithelium. J Biol Chem. 1994; 269(2):1222-1230. (Biology). View Reference
  4. Pin CL, Rukstalis JM, Johnson C, Konieczny SF. The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J Cell Biol. 2001; 155(4):519-530. (Biology: Western blot). View Reference
  5. Zanner R, Hapfelmeier G, Gratzl M, Prinz C. Intracellular signal transduction during gastrin-induced histamine secretion in rat gastric ECL cells. Am J Physiol Cell Physiol. 2002; 282(2):C374-C382. (Biology: Immunofluorescence). View Reference
View All (5) View Less
610313 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.