Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
The eps15 (EGF receptor pathway substrate clone number 15) gene was isolated using an expression cloning method designed for direct isolation of cDNAs encoding substrates of tyrosine kinases. The eps15 gene encodes a 145 kDa protein that is tyrosine phosphorylated following EGF receptor activation and this receptor directly phosphorylates purified Eps15 in vitro. Phosphorylation of Eps15 is much more efficient following EGF receptor activation versus erbB2 kinase activation, suggesting the protein is predominantly a part of an EGF receptor signaling pathway. Overexpression of Eps15 causes transformation of NIH 3T3 cells, implying Eps15 may be involved in the control of cell proliferation. Eps15 binds to the SH3 domain of Crk through its proline-rich domain at the C-terminal region. Adaptin α binds to the C-terminus of Eps15 at three different sites ranging from residues 650 to 730. Eps15, Adaptin α, and EGF receptor are co-localized at the clathrin-coated pits after receptor activation. Therefore, Eps15 may recruit EGF receptors to the clathrin-coated pits for internalization of the activated receptor.
Development References (5)
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Alvarez CV, Shon KJ, Miloso M, Beguinot L. Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps8 and eps15, substrates lacking Src SH2 homology domains. J Biol Chem. 1995; 270(27):16271-16276. (Biology). View Reference
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Engqvist-Goldstein AE, Warren RA, Kessels MM, Keen JH, Heuser J, Drubin DG. The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro. J Cell Biol. 2001; 154(6):1209-1223. (Biology: Immunoprecipitation, Western blot). View Reference
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Iannolo G, Salcini AE, Gaidarov I, et al. Mapping of the molecular determinants involved in the interaction between eps15 and AP-2. Cancer Res. 1997; 57(2):240-245. (Biology). View Reference
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Kariya K, Koyama S, Nakashima S, Oshiro T, Morinaka K, Kikuchi A. Regulation of complex formation of POB1/epsin/adaptor protein complex 2 by mitotic phosphorylation. J Biol Chem. 2000; 275(24):18399-18406. (Biology: Western blot). View Reference
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Traub LM, Downs MA, Westrich JL, Fremont DH. Crystal structure of the alpha appendage of AP-2 reveals a recruitment platform for clathrin-coat assembly. Proc Natl Acad Sci U S A. 1999; 96(16):8907-8912. (Biology: Western blot). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.