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Purified Mouse Anti-Disabled-2/p96
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing), Human, Rat (Tested in Development)
Mouse IgG1
Mouse p96 aa. 31-45
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
96 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610465 Rev. 1
Antibody Details
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CSF-1 is a growth factor that stimulates the growth and differentiation of immature lymphocytes and is required for the survival of mononuclear phagocytes. Binding of CSF-1 induces dimerization and autophosphorylation of its receptor. This results in the activation of several signal transduction pathways. A unique 96 kDa protein is a component in the CSF-1 signal transduction cascade. p96 is phosphorylated on serine following mitogenic stimulation of a mouse macrophage cell line. p96 contains three potential C-terminal ERK kinase phosphorylation sites, as well as several proline-rich sequences that are potential binding sites for SH3-containing proteins. Structural similarities have been found between p96 and Dab, a product of the Drosophila disabled gene, and p96 was also identified as Disabled-2 (Dab-2) and as differentially expressed in ovarian carcinoma-2 (DOC-2) . Dab-2/p96 has been shown to be essential for TGFβ signaling by facilitating signal transduction from the TGFβ receptor to the Smad family of transcription factors. Thus, Dab-2/p96 is an important adaptor molecule in growth factor signaling pathways.

610465 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610465 Rev.1
Citations & References
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Development References (4)

  1. Hocevar BA, Smine A, Xu XX, Howe PH. The adaptor molecule Disabled-2 links the transforming growth factor beta receptors to the Smad pathway. EMBO J. 2001; 20(11):2789-2801. (Clone-specific: Western blot). View Reference
  2. Mishra SK, Keyel PA, Hawryluk MJ, Agostinelli NR, Watkins SC, Traub LM. Disabled-2 exhibits the properties of a cargo-selective endocytic clathrin adaptor. EMBO J. 2002; 21(18):4915-4926. (Clone-specific: Immunofluorescence, Western blot). View Reference
  3. Smith ER, Capo-chichi CD, He J. Disabled-2 mediates c-Fos suppression and the cell growth regulatory activity of retinoic acid in embryonic carcinoma cells. J Biol Chem. 2001; 276(650):47303-47310. (Clone-specific: Western blot). View Reference
  4. Xu XX, Yang W, Jackowski S, Rock CO. Cloning of a novel phosphoprotein regulated by colony-stimulating factor 1 shares a domain with the Drosophila disabled gene product. J Biol Chem. 1995; 270(23):14184-14191. (Biology). View Reference
View All (4) View Less
610465 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.